钼对白消安致小鼠不孕不育症模型繁殖障碍的影响

发布时间：2018-05-24 08:58

本文选题：钼 + 繁殖障碍　；参考：《河南科技大学学报(自然科学版)》2017年01期

【摘要】：为了研究钼对白消安致小鼠不孕不育症模型繁殖障碍的影响,通过单次腹腔注射30 mg/kg白消安构建昆明白小鼠不孕不育症模型。将不孕不育症的雄鼠和雌鼠随机各分为4组,雄鼠饮用水中钼质量浓度为0 mg/L、12.5 mg/L、25.0 mg/L和50.0 mg/L,雌鼠饮用水中钼质量浓度为0 mg/L、2.5 mg/L、5.0 mg/L和10.0 mg/L。饮钼后第1~4周剖取雄鼠睾丸、附睾及雌鼠卵巢,计算附睾及卵巢系数,同时对睾丸及卵巢切片用苏木精-伊红(HE)染色法染色。研究结果表明:雄鼠12.5 mg/L钼组的睾丸形态结构恢复速度较快,附睾系数显著提高,各级精原细胞的层数增多,排列紧密有序;雌鼠2.5 mg/L钼组的卵巢形态结构恢复较好,卵巢内率先出现卵母细胞。钼在适宜剂量12.5 mg/L(雄鼠)或2.5 mg/L(雌鼠)时,对不孕不育小鼠睾丸或卵巢功能有明显恢复作用。
[Abstract]:In order to study the effect of molybdenum on reproductive disorder of mice with sterility induced by Baixiaan, Kunming white mice infertility model was established by single intraperitoneal injection of Baixiaan for 30 mg/kg. Male and female rats with infertility were randomly divided into 4 groups. The molybdenum concentration in drinking water of male rats was 0 mg / L, 12.5 mg / L, 25.0 mg/L and 50.0 mg / L, respectively, and the concentration of molybdenum in drinking water of female rats was 0 mg / L, 2.5 mg / L, 5.0 mg/L and 10.0 mg / L, respectively. The testis, epididymis and ovaries of male and female rats were dissected 4 weeks after molybdenum drinking. The coefficients of epididymis and ovary were calculated, and the testis and ovary sections were stained with hematoxylin and eosin (HEH) staining. The results showed that the recovery rate of testis morphology and structure was faster, the epididymis coefficient was significantly increased, the layers of spermatogonial cells were increased and arranged closely in the 12.5 mg/L molybdenum group of male rats, and the morphological structure of ovary recovered better in 2.5 mg/L molybdenum group. Oocytes are the first to appear in the ovary. Molybdenum at the appropriate dose of 12.5 mg / L (male) or 2.5 mg / L (female) could significantly restore testicular or ovarian function in infertile mice.
【作者单位】：河南科技大学动物科技学院;
【基金】：国家自然科学基金项目(30901026) 河南省高等学校青年骨干教师基金项目(2010GGJS-071)
【分类号】：R711.6;R-332

【相似文献】