龟羚帕安丸对帕金森病大鼠模型中脑黑质细胞Nestin蛋白,Nestin mRNA及DAT蛋白的影响
发布时间:2018-05-24 17:08
本文选题:龟羚帕安丸 + 帕金森病 ; 参考:《中国实验方剂学杂志》2016年04期
【摘要】:目的:观察龟羚帕安丸对帕金森病(PD)大鼠黑质细胞巢蛋白(Nestin),多巴胺转运体(DAT)及Nestin mRNA表达的影响。方法:大鼠黑质致密部注射6-羟基多巴胺(6-OHDA),后大鼠ip阿扑吗啡(APO)诱导旋转建立PD大鼠模型。PD大鼠随机分为模型组,多巴丝肼组,龟羚帕安丸高、中、低剂量组,同时设立假手术组,模型组。假手术组每日ig同等容积的生理盐水;多巴丝肼组ig多巴丝肼混悬液(10 mg·kg~(-1)·d~(-1));龟羚帕安丸高、中、低剂量组ig龟羚帕安丸混悬液(4,2,1 g·kg~(-1)·d~(-1)),连续30 d。免疫组化法检测各组大鼠中脑黑质Nestin,DAT蛋白表达,原位杂交法检测各组大鼠中脑黑质Nestin mRNA的表达。结果:模型组黑质细胞Nestin,DAT蛋白,Nestin mRNA表达均显著低于假手术组(P0.01);各治疗组Nestin蛋白,Nestin mRNA,DAT蛋白表达均显著高于模型组(P0.01)。结论:龟羚帕安丸治疗帕金森病的作用机制可能与上调Nestin,DAT表达,促进神经的修复和再生,增加多巴胺(DA)含量有关。
[Abstract]:Aim: to observe the effect of Guilingpaan pill on the expression of nestin, dopamine transporter (DATT) and Nestin mRNA in substantia nigra of rats with Parkinson's disease (PD). Methods: PD rat model was established by injecting 6-hydroxydopamine (6-OHDAA) into the substantia nigra compactly and then by IP apomorphine (APO). PD rats were randomly divided into three groups: model group, dobutazide group, high, middle and low dose group of Guilingpaan pills. At the same time, set up sham operation group, model group. The sham operation group was given the same volume of normal saline daily; the dobutazid group was given intravenously with dobutazid suspension of 10 mg kg 路kg ~ (-1) 路d ~ (-1) ~ (-1) d ~ (-1); the group of Guilingpaan pills was high, medium and low dose group, and the group of ig was given 4 ~ 2g / kg / kg ~ (-1) 路d ~ (-1) 路d ~ (-1) ~ (-1) of the suspension of Guilingpaan Pill (ig) for 30 days. The expression of Nestin mRNA in substantia nigra was detected by immunohistochemistry and in situ hybridization was used to detect the expression of Nestin mRNA in substantia nigra. Results: the expression of nestin mRNA in substantia nigra cells in the model group was significantly lower than that in the sham-operated group, and the expression of nestin mRNA-DAT protein in all treatment groups was significantly higher than that in the model group. Conclusion: the mechanism of Guilingpaan Pill in the treatment of Parkinson's disease may be related to the up-regulation of Nestinine DAT expression, the promotion of nerve repair and regeneration, and the increase of dopamine (DA) content.
【作者单位】: 河南中医药大学基础医学院;河南中医药大学第二临床医学院;河南中医药大学;
【基金】:河南省中医管理局中医临床学科领军人才培育计划项目(201301006) 河南中医学院科技创新人才支持计划项目(2010XCXRC06)
【分类号】:R285.5;R-332
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