小柴胡汤对伊立替康致迟发性腹泻模型小鼠血便的防治作用

发布时间：2018-05-25 04:03

本文选题：小柴胡汤 + 伊立替康　；参考：《中国药房》2017年13期

【摘要】：目的:考察小柴胡汤对伊立替康(CPT-11)致迟发性腹泻模型小鼠血便的防治作用。方法:将40只小鼠随机分为正常对照组(等体积生理盐水)、模型对照组(等体积生理盐水)、小柴胡汤组(1 500 mg/kg,以生药计)和洛哌丁胺组(阳性对照,0.533 3mg/kg),ig给药,每天1次,连续17 d。于实验第4~10天,除正常对照组外,其余各组小鼠均ip CPT-11诱导迟发性腹泻。测定小鼠体质量变化、血便率并观察小鼠大肠、小肠黏膜组织病理改变。结果:给药17 d后,造模组小鼠的体质量较正常对照组均明显下降(P0.05),但小柴胡汤组小鼠体质量高于模型对照组(P0.05)。实验第11~17天,小柴胡汤组和洛哌丁胺组小鼠血便率均显著低于模型对照组(P0.01),且小柴胡汤组小鼠血便率低于洛哌丁胺组(P0.05)。病理观察结果显示,模型对照组小鼠大肠和小肠黏膜上皮细胞伴明显弥漫性坏死脱落和炎症细胞浸润,给药组小鼠肠黏膜组织均趋于正常。结论:小柴胡汤能有效降低血便率、改善肠黏膜组织损伤,对CPT-11致小鼠血便具有防治作用,且效果优于阳性药物洛哌丁胺。
[Abstract]:Aim: to investigate the preventive and therapeutic effects of Xiaochaihu decoction on blood stool in mice with delayed diarrhea induced by Iritekang (CPT-11). Methods: forty mice were randomly divided into normal control group (equal volume of normal saline), model control group (equal volume of normal saline), Xiaochaihu decoction group (1 500 mg / kg, based on crude drugs) and ropipramine group (positive control group, 0. 533 mg / kg 路kg ~ (-1) ig, once a day). In a row of 17 days. On the 4th day of the experiment, delayed diarrhea was induced by IP CPT-11 in all mice except the normal control group. The changes of body mass, blood stool rate and the pathological changes of large intestine and small intestine were observed. Results: after 17 days of administration, the body weight of mice in the model group was significantly lower than that in the normal control group, but the body weight of mice in the Xiaochaihu decoction group was higher than that in the model control group. On the 11th 17th day of experiment, the blood stool rate of mice in Xiaochaihu decoction group and ropipramine group was significantly lower than that in model control group (P 0.01), and the blood stool rate in Xiao Chai Hu decoction group was lower than that in loperamine group (P 0.05). The results of pathological observation showed that the epithelial cells of large intestine and small intestine in the model control group were obviously diffuse necrosis and exfoliation and inflammatory cell infiltration. Conclusion: Xiaochaihu decoction can effectively reduce blood stool rate, improve intestinal mucosal tissue injury, and can prevent and treat blood stool of CPT-11 mice, and the effect is superior to that of ropipramine positive drug.
【作者单位】：贵州医科大学药理教研室;十堰市太和医院武当中医药研究所;
【基金】：湖北省卫生和计划生育委员会2017-2018年度科研立项项目(No.WJ2017F079)
【分类号】：R285.5;R-332

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