IGF-Ⅰ与MGF对骨骼肌卫星细胞增殖及迁移的影响

发布时间：2018-05-26 05:07

本文选题：骨骼肌卫星细胞 + 机械生长因子　；参考：《上海体育学院》2011年硕士论文

【摘要】：MGF组:24h时,15ng/ml组、25ng/ml组、35ng/ml组和45ng/ml组OD值均显著高于对照组(P0.01,P0.01,P0.01,P0.05),55ng/ml组与对照组无显著差异(P0.05)。48h时,15ng/ml组、25ng/ml组、35ng/ml组OD值均显著高于对照组(P0.05,P0.01,P0.01), 45ng/ml组、55ng/ml组与对照组之间无显著差异(P0.05)。72h时,15ng/ml、25ng/ml组、35ng/ml组OD值均显著高于对照组(P0.01),45ng/ml组与对照组之间无显著差异(P0.05)。而55ng/ml组OD值低于对照组(P0.05)。96h时,15ng/ml、25ng/ml组、35ng/ml组与对照组之间差异没有显著性(P0.05)。 4. Transwell法测定细胞迁移的OD值显示。 IGF-I组:15ng/ml组、35ng/ml组与对照组之间的无显著差异(P0.05);25ng/ml、45ng/ml组、55ng/ml组OD值均显著高于对照组(P0.01),其中25ng/ml组显著高于45ng/ml组(P0.01),55ng/ml组与45ng/ml组之间的无显著差异(P0.05)。 MGF组:15ng/ml组、35ng/ml组与对照组之间无显著差异(P0.05);25ng/ml、45ng/ml组、55ng/ml组OD值显著高于对照组(P0.01),其中45ng/ml组和55ng/ml组均显著高于25ng/ml组(P0.01,P0.05)。结论 1. IGF-I和MGF均可以促进骨骼肌卫星细胞增殖且呈浓度、时间依赖性,IGF-I最佳促增殖浓度为15ng/ml-35ng/ml , MGF最佳促增殖浓度为25ng/ml-35ng/ml,干预24h后即开始增殖。 2. IGF-I和MGF均可以促进骨骼肌卫星细胞迁移,IGF-I促迁移的最佳浓度为25ng/ml,MGF最佳浓度为45ng/ml-55ng/ml。MGF组:24h时,15ng/ml组、25ng/ml组、35ng/ml组和45ng/ml组OD值均显著高于对照组(P0.01,P0.01,P0.01,P0.05),55ng/ml组与对照组无显著差异(P0.05)。48h时,15ng/ml组、25ng/ml组、35ng/ml组OD值均显著高于对照组(P0.05,P0.01,P0.01), 45ng/ml组、55ng/ml组与对照组之间无显著差异(P0.05)。72h时,15ng/ml、25ng/ml组、35ng/ml组OD值均显著高于对照组(P0.01),45ng/ml组与对照组之间无显著差异(P0.05)。而55ng/ml组OD值低于对照组(P0.05)。96h时,15ng/ml、25ng/ml组、35ng/ml组与对照组之间差异没有显著性(P0.05)。 4. Transwell法测定细胞迁移的OD值显示。 IGF-I组:15ng/ml组、35ng/ml组与对照组之间的无显著差异(P0.05);25ng/ml、45ng/ml组、55ng/ml组OD值均显著高于对照组(P0.01),其中25ng/ml组显著高于45ng/ml组(P0.01),55ng/ml组与45ng/ml组之间的无显著差异(P0.05)。 MGF组:15ng/ml组、35ng/ml组与对照组之间无显著差异(P0.05);25ng/ml、45ng/ml组、55ng/ml组OD值显著高于对照组(P0.01),其中45ng/ml组和55ng/ml组均显著高于25ng/ml组(P0.01,P0.05)。结论 1. IGF-I和MGF均可以促进骨骼肌卫星细胞增殖且呈浓度、时间依赖性,IGF-I最佳促增殖浓度为15ng/ml-35ng/ml , MGF最佳促增殖浓度为25ng/ml-35ng/ml,干预24h后即开始增殖。 2. IGF-I和MGF均可以促进骨骼肌卫星细胞迁移,IGF-I促迁移的最佳浓度为25ng/ml,MGF最佳浓度为45ng/ml-55ng/ml。
[Abstract]:The OD value of MGF group was significantly higher than that of control group P0.01ngpml group and 45ng/ml group at 24 hours. There was no significant difference between the control group and the control group. The OD value of the 15ng / ml group was significantly higher than that of the control group (P0.05ngP / ml group, P0.05g / ml group and control group, P0.05ng / ml group and control group at 2h / 48h). The OD value of the 15ng / ml group was significantly higher than that of the control group (P0.05g / ml group and control group). There was no significant difference between the 45ng/ml group and the control group (P0.05g / ml group and the control group at 2h. 7 h). The OD value of the 15ng / ml group was significantly higher than that of the control group (P0.05g / ml group and control group). The OD value of 15ng / ml 25ng / ml group was significantly higher than that of control group (P0.01ng / ml) and control group (P0.05ng-1 / ml), and there was no significant difference between control group and control group (P0.05ng / ml). However, the OD value of 55ng/ml group was significantly lower than that of control group (P 0.05) at 96 h. There was no significant difference between the 35 ng / ml group and the control group at the time of 15 ng / ml or 25 ng / ml. 4. OD value of cell migration was measured by Transwell method. There was no significant difference between the IGF-I group and the control group. The OD value of the 55ng / ml group was significantly higher than that of the control group (P0.05ng-1 / ml). The OD value of the 25ng/ml group was significantly higher than that of the 45ng/ml group (P0.01ngr / ml group) and the 45ng/ml group (P0.05N). There was no significant difference between the MGF group and the control group. The OD value of the 55ng / ml group was significantly higher than that of the control group (P 0.05ng / ml). The OD values of the 45ng/ml group and the 55ng/ml group were significantly higher than that of the 25ng/ml group (P0.01P0.05). Conclusion 1. Both IGF-I and MGF could promote the proliferation of skeletal muscle satellite cells. The best concentration of IGF-I was 15ng/ml-35ng/ml, and the best concentration of MGF was 25ng / ml-35ng / ml. After 24 hours of intervention, the proliferation began. 2. IGF-I and MGF can promote the migration of skeletal muscle satellite cells. The best concentration of IGF-I to promote migration of skeletal muscle satellite cells is 25ng / ml MGF at 24 hours in 45ng/ml-55ng/ml.MGF group. The OD value of 25ng / ml group and 45ng/ml group is significantly higher than that of control group P0.01P0.01P0.01P0.01P0.01P0.01P0.01P0.01P0.01P0.05ngP / ml at 48h. There is no significant difference between control group and control group (P0.05g / ml, 48h). The OD value of the 35ng / ml group was significantly higher than that of the control group (P0.05ng-1 / ml). There was no significant difference between the 45ng/ml group and the control group (P0.05g / ml). There was no significant difference between the 45ng/ml group and the control group at 72h. The OD value of the 35ng / ml group was significantly higher than that of the control group (P0.01ngml group and control group). There was no significant difference between the control group and the control group. However, the OD value of 55ng/ml group was significantly lower than that of control group (P 0.05) at 96 h. There was no significant difference between the 35 ng / ml group and the control group at the time of 15 ng / ml or 25 ng / ml. 4. OD value of cell migration was measured by Transwell method. There was no significant difference between the IGF-I group and the control group. The OD value of the 55ng / ml group was significantly higher than that of the control group (P0.05ng-1 / ml). The OD value of the 25ng/ml group was significantly higher than that of the 45ng/ml group (P0.01ngr / ml group) and the 45ng/ml group (P0.05N). There was no significant difference between the MGF group and the control group. The OD value of the 55ng / ml group was significantly higher than that of the control group (P 0.05ng / ml). The OD values of the 45ng/ml group and the 55ng/ml group were significantly higher than that of the 25ng/ml group (P0.01P0.05). Conclusion 1. Both IGF-I and MGF could promote the proliferation of skeletal muscle satellite cells. The best concentration of IGF-I was 15ng/ml-35ng/ml, and the best concentration of MGF was 25ng / ml-35ng / ml. After 24 hours of intervention, the proliferation began. 2. Both IGF-I and MGF could promote the migration of skeletal muscle satellite cells. The best concentration of IGF-I was 25ng / ml ml-55ng / ml.
【学位授予单位】：上海体育学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R329

【引证文献】