2009甲型H1N1流感病毒与普通季节性流感病毒感染细胞效率的比较

发布时间：2018-05-26 22:27

本文选题：甲型流感病毒 + 季节性流感　；参考：《北京协和医学院》2012年硕士论文

【摘要】：2009年4月在墨西哥爆发了一种新型的甲型流感病毒(Novel Swine-origin Influenza A Virus, S-OIV)并迅速传播至全世界许多国家和地区,给当地的生产生活造成严重影响。2009年6月11日,世界卫生组织(WHO)宣布将此次流感病毒的全球警戒水平提升至6级,这也预示着全球流感大流行的爆发。虽然此次甲型H1N1流感病毒感染者多数为散在个体,并且感染症状比较轻微,但对于青年人以及那些本来患有哮喘、糖尿病、病态肥胖症的人群以及孕妇等来说,由此引起更严重疾病的可能性将会大大增加。根据WHO最新统计结果,一般情况下因感染季节性流感而死亡的人数每年都在25-50万之间。此次甲型H1N1流感截至2010年8月共导致1.8万人死亡,死亡率为4%。健康保护机构(HPA)预言此次甲型H1N1可能会卷土重来。 2009甲型H1N1流感病毒含有源自人类、猪以及禽甲型H1N1流感基因,为三种不同来源的甲型病毒的重配株。它在病毒包装过程中,极易将不同来源的基因片段组合在一起,成为不同来源流感病毒的“混合器”,因此与季节性甲型流感病毒相比,2009甲型H1N1流感病毒更容易逃逸人体免疫系统的监视,造成更严重的流感大流行的爆发。我们发现,在A549细胞中,季节性H1N1流感病毒的感染效率明显高于2009甲型H1N1流感病毒；而在H1650细胞中,2009甲型H1N1流感病毒的感染效率明显高于季节性H1N1流感病毒。我们同时也证实了H1N1病毒进入细胞依赖于clathrin介导的内吞方式。
[Abstract]:The outbreak in Mexico in April 2009 of a new influenza A virus, Novel Swine-origin Influenza A Virus, S-OIV), which spread rapidly to many countries and regions around the world, with a serious impact on local production and life. 11 June 2009, The World Health Organization (WHO) announced that it would raise the global alert level to phase 6, heralding an outbreak of a global influenza pandemic. Although most of the people infected with the influenza A H1N1 virus are scattered in individuals and the symptoms of the infection are mild, it is for young people and those who are already suffering from asthma, diabetes, morbid obesity and pregnant women. The likelihood of causing more serious illness will be greatly increased. According to the latest WHO statistics, the average number of deaths from seasonal flu is between 25 to 500, 000 a year. As of August 2010, a total of 18000 people had died from the H1N1 flu, with a mortality rate of 4. HPA, a health protection agency, predicted that the A-A H1N1 might come back. The 2009 H1N1 influenza virus contains genes derived from human, pig and avian influenza A, and is a reassortment of three different sources of A virus. In the process of virus packaging, it is easy to combine gene fragments from different sources and become a "mixer" of influenza viruses from different sources. Therefore, compared with seasonal influenza A virus, 2009 H1N1 influenza virus is easier to escape the surveillance of human immune system, resulting in a more serious outbreak of influenza pandemic. We found that the infection efficiency of seasonal H1N1 influenza virus was significantly higher than that of 2009 A H1N1 influenza virus in A549 cells, while the infection efficiency of 2009 H1N1 influenza virus in H1650 cells was significantly higher than that of seasonal H1N1 influenza virus. We also confirmed that H1N1 virus enters cells dependent on clathrin mediated endocytosis.
【学位授予单位】：北京协和医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R373

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