慢性噪音暴露诱发小鼠低焦虑样行为及其分子机制的研究

发布时间：2018-05-27 05:07

本文选题：噪音暴露 + 低焦虑　；参考：《复旦学报(自然科学版)》2011年03期

【摘要】：噪音日益严重地影响着人类的健康,为了探讨慢性噪音对小鼠情绪和注意力的影响及其分子机制.分别给予6周龄雄性小鼠以噪音刺激(75 dB,8 h/d),连续30 d后检测小鼠与情绪和注意力相关的行为表现.结果发现,噪音组小鼠在开场实验中心区域运动距离的百分比升高,在明暗箱实验中表现为在明箱中运动时间增多以及在强迫游泳实验中静止不动的时间显著减少,均与对照组有显著差异.在痕迹恐惧实验中,两组小鼠在线索式恐惧测试中的僵直程度相似,说明持续的声音暴露没有影响小鼠的注意力.运用荧光定量PCR技术检测海马和下丘脑相关基因的表达,发现噪音组小鼠GAD65 mRNA水平、GABAAα1/GABAAα3比例和NR2A/NR2B的比例均下降,且NR1 mRNA表达量上调.以上结果显示,慢性持久的噪音暴露使小鼠产生低焦虑样行为,其分子机制可能涉及谷氨酸能兴奋性神经元活动增强,以及γ-氨基丁酸能神经元对大脑活动抑制功能的减弱.
[Abstract]:In order to study the effects of chronic noise on emotion and attention of mice and its molecular mechanism, noise affects human health more and more seriously. 6-week-old male mice were given noise stimulation of 75 dB / d for 8 h / d respectively. After 30 days, the behavior related to emotion and attention was measured. The results showed that the percentage of exercise distance in the center of the open field was increased in the noise group, and the time of exercise in the open box and the time of immobility in the forced swimming test were significantly decreased in the light and dark box experiment. There was significant difference between the two groups. In the trace fear test, the degree of rigidity of the two groups was similar in the cue-type fear test, indicating that the sustained sound exposure did not affect the attention of the mice. Fluorescence quantitative PCR technique was used to detect the expression of genes related to hippocampus and hypothalamus. It was found that the ratio of GABA AA 伪 1/GABAA 伪 3 and NR2A/NR2B in GAD65 mRNA level of noise group decreased, and NR1 mRNA expression was up-regulated. These results suggest that chronic and persistent noise exposure may lead to low anxiety like behavior in mice, and its molecular mechanism may be related to the increased activity of glutamate excitatory neurons and the decrease of the inhibitory function of 纬 -aminobutyric acid neurons on brain activity.
【作者单位】：华东师范大学教育部及上海市脑功能基因组学重点实验室;
【基金】：华东师范大学科研创新基金资助项目
【分类号】：R363

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