甲状腺素对大鼠成骨细胞增殖及碱性磷酸酶活力和骨钙素含量的影响

发布时间：2018-05-27 11:11

本文选题：甲状腺素 + 成骨细胞　；参考：《环境与健康杂志》2017年01期

【摘要】：为探讨甲状腺素(T_4)对体外培养初生乳鼠颅骨成骨细胞增殖及碱性磷酸酶(ALP)活力和骨钙素(OC)含量的影响,采用酶消化法培养原代新生SD大鼠颅骨成骨细胞,分别暴露于含终浓度为0(对照)、10~(-9)、10~(-8)、10~(-7) mol/L T_4的培养基培养24、48、72 h,观察成骨细胞的增殖情况;染毒结束后,检测细胞ALP活力和OC含量。结果显示,与对照组比较,10~(-8)、10~(-7)mol/L T_4暴露24、48、72 h后成骨细胞的存活率均较高,差异有统计学意义(P0.05);而10~(-9) mol/L T_4暴露组成骨细胞的存活率无明显改变。且随着T_4暴露浓度的升高和暴露时间的延长,成骨细胞的存活率均呈上升趋势。与对照组比较,10~(-8)、10~(-7)mol/L T_4暴露组成骨细胞的ALP活力和OC含量均较高,差异均有统计学意义(P0.05);而10~(-9) mol/L T_4暴露组成骨细胞的ALP活力及OC含量均无明显改变。且随着T_4暴露浓度的升高,成骨细胞的ALP活力和OC含量均呈上升趋势,提示T_4具有一定的促成骨作用。
[Abstract]:In order to investigate the effect of TX _ 4 on the osteoblast proliferation, alkaline phosphatase (ALP) activity and osteocalcin (OC) content of calvarial osteoblasts of newborn rats in vitro, osteoblasts from the skull of newborn SD rats were cultured by enzyme digestion. The proliferation of osteoblasts was observed in the culture medium containing mol/L T4 containing a final concentration of 0 (control group 10, 10, 10, 10, 10, 10), and the activity of ALP and the content of OC, respectively, after exposure to the culture medium for 24 ~ 4872 h. The results showed that compared with the control group, the survival rate of osteoblasts was higher in the 10 ~ 10 ~ (-7) mol 路L ~ (-1) T _ (4) group than that in the control group (P < 0.05), but there was no significant change in the survival rate of osteoblasts in the mol/L T4 exposure group. The survival rate of osteoblasts increased with the increase of T4 exposure concentration and the prolongation of exposure time. Compared with the control group, the ALP activity and OC content in the osteoblast exposed to 10 ~ 10 ~ (-7) mol / L ~ (-1) T _ (4) were higher than those in the control group (P 0.05), while the ALP activity and OC content in the osteoblasts exposed to mol/L T _ 4 had no significant change. The ALP activity and OC content of osteoblasts increased with the increase of T _ 4 exposure concentration, which suggested that T _ P _ 4 could promote the bone of osteoblasts.
【作者单位】：沧州医学高等专科学校医学系;武警8640部队医院院务办公室;沧州市传染病医院医务科;泊头市人民医院心血管内科;
【基金】：河北省沧州市科学技术研究与发展指导计划项目(08ZD235)
【分类号】：R329.2

【相似文献】