慢性复合应激对家兔多个系统功能的影响及Orcu-DRB等位基因的测定

发布时间：2018-05-29 01:53

本文选题：应激 + 动物模型　；参考：《大连医科大学》2012年硕士论文

【摘要】：目的：本文通过对慢性复合型应激状态下家兔的体重、生存时间、血清糖皮质激素、生化指标水平、抗鸡卵核抗体和总抗氧化能力等指标的观察，探讨慢性复合型应激对家兔多个系统功能状态的影响。方法：由束缚、浸水、隔离限制三种应激方式共同组成而建立本次慢性复合型应激动物模型，并从以下三个方面来研究慢性复合型应激对家兔产生的影响。 1、在实施慢性复合型应激的过程中，记录家兔每日体重变化、存活数与死亡数，通过分析慢性复合型应激后较应激前家兔的体重变化，并在后期进行生存分析，统计累积生存率，分析应激时间与家兔累积生存率的关系，并由此对本实验动物模型的建立进行评价。 2、通过检测慢性复合应激前后家兔血清中总蛋白、白蛋白、葡萄糖、谷丙转氨酶、肌酐及乳酸脱氢酶等常见临床生化指标以及糖皮质激素、抗鸡卵核抗体及总抗氧化能力的水平，研究慢性复合型应激对上述各项指标的影响。 3、取用家兔耳缘动脉血，提取全血基因组DNA，，采用PCR-SSP技术检测以Orcu-DRB*02型等位基因为代表的兔DRB等位基因，并对家兔携带与未携带Orcu-DRB*02型等位基因者糖皮质激素及体重变化进行了初步的分析。结果： 1、通过观察分析应激后较应激前家兔的体重变化，发现应激后家兔的体重显著性下降，并于应激第31天达到最低点。在应激第4天时，应激组家兔发生第一例死亡。在应激第15天时，累积生存率为50%，并且家兔累计生存率在应激后呈持续显著性下降趋势。 2、应激后尤其在第四周家兔血清中的葡萄糖较应激前相比显著下降，应激后的血清总抗氧化能力较应激前相比降低；应激后家兔体内的糖皮质激素水平较应激前有显著增高。而应激后血清中总蛋白、球蛋白、白蛋白、谷丙转氨酶、谷草转氨酶、GGT、肌酐、乳酸脱氢酶、抗鸡卵核抗体与应激前相比没有显著性差异。 3、Orcu-DRB等位基因检测结果：36只家兔中，Orcu-DRB*02型等位基因阳性表达的家兔有3只，阳性率为8.33%。未携带Orcu-DRB*02型等位基因的家兔体重随着应激时间的增加而有上升的趋势，而携带Orcu-DRB*02型等位基因的家兔体重随着应激时间的增加而有下降的趋势。结论： 1.慢性复合应激后家兔的体重增长明显受抑，说明此次建立动物模型的方法有效。 2.慢性复合应激后家兔血清中的葡萄糖较应激前相比有显著下降；应激后的血清总抗氧化能力较应激前相比降低，说明葡萄糖、总抗氧化能力这两项指标对慢性应激比较敏感。 3.在新西兰兔中可以检测出Orcu-DRB*02型等位基因。
[Abstract]:Objective: to observe the body weight, survival time, serum glucocorticoid, biochemical index, anti-oocyte antibody and total antioxidant ability of rabbits under chronic complex stress. To investigate the effects of chronic complex stress on the functional states of multiple systems in rabbits. Methods: the animal model of chronic compound stress was established by three stress modes: restraint, immersion and isolation. The effects of chronic compound stress on rabbits were studied from the following three aspects. 1. In the course of implementing chronic compound stress, the changes of daily weight, the number of survival and the number of deaths in rabbits were recorded. The changes of weight of rabbits after chronic compound stress were analyzed compared with those before stress, and survival analysis was carried out at the later stage. The relationship between the stress time and the cumulative survival rate of rabbits was analyzed, and the establishment of the animal model was evaluated. 2. Serum total protein, albumin, glucose, alanine aminotransferase, creatinine, lactate dehydrogenase and glucocorticoid in serum of rabbits before and after chronic multiple stress were detected. To study the effect of chronic complex stress on the above indexes in the level of anti-oocyte antibody and total antioxidant capacity. 3. The genomic DNA was extracted from rabbit auricular artery blood, and the DRB alleles represented by Orcu-DRB*02 type allele were detected by PCR-SSP technique. The changes of glucocorticoid and body weight in rabbits with and without Orcu-DRB*02 alleles were analyzed. Results: 1. By observing and analyzing the weight changes of rabbits after stress, it was found that the weight of rabbits decreased significantly after stress, and reached the lowest point on the 31st day of stress. On the fourth day of stress, the first death occurred in the stress group. On the 15th day of stress, the cumulative survival rate was 50%, and the cumulative survival rate of rabbits continued to decrease significantly after stress. (2) after stress, especially in the fourth week, the serum glucose decreased significantly, and the total antioxidation capacity of serum decreased after stress, and the level of glucocorticoid in rabbits after stress was significantly higher than that before stress. However, there was no significant difference in serum total protein, globulin, albumin, alanine aminotransferase, glutamic oxaloacetic transaminase (GGTs), creatinine, lactate dehydrogenase and anti-oocyte antibody. 3The results of Orcu-DRB allele analysis showed that there were 3 rabbits with positive expression of Orcu-DRBF02 allele in 36 rabbits, and the positive rate was 8.33%. The weight of rabbits without Orcu-DRB*02 allele increased with the increase of stress time, while the weight of rabbits with Orcu-DRB*02 allele decreased with the increase of stress time. Conclusion: 1. The weight gain of rabbits was significantly inhibited after chronic multiple stress, which indicated that the method of establishing animal model was effective. 2. The total antioxidant capacity of serum after stress was lower than that before stress, which indicated that glucose and total antioxidant capacity were sensitive to chronic stress. 3. The Orcu-DRB*02 allele can be detected in New Zealand rabbits.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363;R3416

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