非人灵长类评价学习记忆功能的影响因素探讨

发布时间：2018-05-29 07:20

本文选题：非人灵长类 + 学习记忆　；参考：《中国比较医学杂志》2017年09期

【摘要】：目的基于威斯康辛通用测试仪的延迟应答测试是评价非人灵长类学习记忆能力的常用方法,该实验在进行过程中受到很多因素影响,如何设定规范的训练和评价参数,对正确评价猴的学习记忆能力具有重要的参考意义和价值。方法本研究选取8~9岁雄性食蟹猴作为实验研究对象,利用改良的威斯康辛通用测试仪检测,通过食蟹猴获取食物的正确率来评价训练次数,评价频率,间隔评价时间,延迟时间和陷阱食盒数量等实验因素对结果的影响。结果当减少训练阶段的训练次数(小于10次)时,食蟹猴获取食物的正确率不稳定且呈现出显著的下降趋势;在获得足够训练的前提下,评价频率对食蟹猴获取食物的正确率影响较小;常规训练后3个月进行评价,食蟹猴仍然能够获得65%的获取食物正确率;增加延迟时间后,会导致部分食蟹猴获取食物的正确率显著降低;此外,当增加到3个以上陷阱食盒时,导致食蟹猴获取食物正确率普遍低于65%以下。结论合理的设置训练和评价阶段的实验参数对非人灵长类学习记忆功能的评价具有重要的影响和意义。
[Abstract]:Objective to evaluate the learning and memory ability of non-human primates, the delayed response test based on the Wisconsin universal tester is a common method. The experiment is affected by many factors, and how to set the standard training and evaluation parameters. It has important reference significance and value for correctly evaluating the ability of learning and memory of monkey. Methods in this study, 8- and 9-year-old male crab-eating monkeys were selected as the experimental objects. The modified Wisconsin universal testing instrument was used to evaluate the training times, the frequency and the interval evaluation time by using the correct rate of obtaining food for the crab-eating monkeys. The effects of delay time and the number of trap food boxes on the results. Results when the number of times of training (less than 10 times) was reduced, the correct rate of obtaining food was unstable and showed a significant downward trend. The frequency of evaluation had little effect on the correct rate of obtaining food. After 3 months of routine training, the correct rate of obtaining food was still 65%. In addition, when increased to more than 3 trap food boxes, the accuracy rate of obtaining food is generally lower than 65%. Conclusion the reasonable setting of experimental parameters in training and evaluation stage has important influence and significance on the evaluation of learning and memory function of non-human primates.
【作者单位】：广东省实验动物重点实验室广东省实验动物监测所;中山大学附属第一医院神经科;
【基金】：广东省科技计划项目(编号:2014B040404053,2015A020212027)
【分类号】：Q42;R-33

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