脾气虚模型大鼠海马与下丘脑神经元线粒体分裂因子和线粒体分裂蛋白1的表达

发布时间：2018-05-29 23:30

本文选题：脾气虚 + 线粒体　；参考：《中国中西医结合杂志》2017年11期

【摘要】：目的观察脾气虚模型大鼠海马、下丘脑神经元线粒体形态学变化及其分裂情况,探讨脾气虚的发生机制。方法 20只SPF级雄性SD大鼠随机分为非脾气虚组和脾气虚模型组,每组10只。脾气虚模型组采用饮食失节+劳倦方法制脾气虚模型。透射电镜观察海马CA1区及下丘脑神经元线粒体的形态学变化;ELISA法检测海马和下丘脑组织ATP含量;Western blot法检测上述组织线粒体分裂因子(MFF)和线粒体分裂蛋白1(Fis1)的表达。结果非脾气虚组线粒体较丰富,形态基本正常,而脾气虚模型组线粒体数量减少,其中海马CA1区神经元线粒体出现肿胀、嵴减少,甚至空泡化,而下丘脑还存在较多线粒体自噬现象。与非脾气虚组比较,脾气虚模型组体重、进食量、饮水量、运动距离、站立次数以及前肢抓力均下降,海马和下丘脑神经元ATP含量降低,MFF及Fis1蛋白表达升高(P0.05,P0.01)。结论海马和下丘脑相关神经元线粒体功能低下及其促分裂成分表达增加与脾气虚的发生密切相关。
[Abstract]:Objective to observe the morphological changes of mitochondria and division of hippocampal and hypothalamic neurons in rats with spleen qi deficiency and to explore the mechanism of spleen qi deficiency. Methods Twenty SPF male Sprague-Dawley rats were randomly divided into non-spleen Qi deficiency group and spleen Qi deficiency model group with 10 rats in each group. The spleen qi deficiency model group was made by the method of dietetic exhaustion. The morphological changes of mitochondria in hippocampal CA1 and hypothalamic neurons were observed by transmission electron microscope. The ATP content in hippocampus and hypothalamus was detected by Elisa and the expression of Mitochondrial cleavage Factor (MFF) and Mitochondrial Mitochondrial Mitochondrial protein (1 / Fis1) were detected by Western blot. Results the mitochondria were abundant and normal in non-spleen qi deficiency group, but the number of mitochondria in spleen qi deficiency model group was decreased. The mitochondria in hippocampus CA1 area were swollen, cristae decreased and even vacuolated. The hypothalamus also has more mitochondrial autophagy. Compared with the non-spleen qi deficiency group, the body weight, food intake, drinking water, exercise distance, standing times and forelimb grasping power of the spleen qi deficiency model group were all decreased, and the ATP contents of hippocampus and hypothalamus neurons were decreased. The expression of MFF and Fis1 protein was increased in the hippocampal and hypothalamic neurons. Conclusion the decrease of mitochondrial function and the increased expression of mitogenic components in hippocampal and hypothalamus-related neurons are closely related to the occurrence of spleen qi deficiency.
【作者单位】：辽宁中医药大学教学实验中心机能实验室;辽宁中医药大学研究生学院;辽宁中医药大学中医脏象理论及应用教育部重点实验室;辽宁中医药大学基础医学院生理与心理教研室;
【基金】：国家重点基础研究发展计划(No.2013CB531702) 国家自然科学基金资助项目(No.81673851)
【分类号】：R228;R-332

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