当归补血汤对心肌缺血模型大鼠心肌组织氧化应激的影响

发布时间：2018-05-30 09:54

本文选题：当归补血汤 + 心肌缺血　；参考：《时珍国医国药》2016年08期

【摘要】：目的研究当归补血汤在冠脉结扎诱导的大鼠心肌缺血模型中的抗氧化活性。方法将75只健康SD大鼠随机分为5组,分别为假手术组,模型组,地尔硫卓(10 mg·kg~(-1))组,当归补血汤(3 g·kg~(-1))组,当归补血汤(6 g·kg~(-1))组,连续给药7天后,行冠脉左前降支结扎术制备大鼠心肌缺血模型,24h后,采用HE染色检测心肌病理学改变情况;测定各组血清中的白介素(IL)-6,肿瘤坏死因子(TNF)-α,超氧化物歧化酶(SOD),丙二醛(MDA),过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPx);采用Western Blot检测大鼠心脏组织氧化应激相关蛋白表达水平。结果当归补血汤(3 g·kg-1,6 g·kg~(-1))能显著改善冠脉结扎诱导的心脏病理学改变;明显降低冠脉结扎诱导的心肌缺血大鼠血清TNF-α、IL-6和MDA的含量;提高血清中SOD、CAT和GPx的活性含量;同时,在当归补血汤的干预下,氧化应激相关蛋白的SOD1、CAT和GPx的表达明显上调,而MDA5蛋白表达明显下调。结论当归补血汤对冠脉结扎致大鼠心肌缺血有保护作用,其作用可能与调节氧化应激相关细胞因子含量及蛋白表达水平有关。
[Abstract]:Objective to study the anti-oxidation activity of Danggui Buxue decoction (Danggui Buxue decoction) in myocardial ischemia induced by coronary artery ligation in rats. Methods Seventy-five healthy Sprague-Dawley rats were randomly divided into five groups: sham operation group, model group, diltiazem (10 mg / kg) group, Danggui Buxue decoction (3 g / kg) group and Danggui Buxue decoction (6 g / kg) group (n = 7). 24 hours after left anterior descending coronary artery ligation, myocardial pathological changes were detected by HE staining. The levels of IL-6, TNF- 伪, SOD, MDAA, Glutathione peroxidase (Glutathione peroxidase) and Glutathione peroxidase (Glutathione peroxidase) in the serum of each group were determined by Western Blot. Results Danggui Buxue decoction (3 g 路kg ~ (-1) could significantly improve the cardiac pathological changes induced by coronary artery ligation, decrease the contents of serum TNF- 伪 IL-6 and MDA, increase the activities of cat and GPx in serum, at the same time, increase the content of TNF- 伪 -IL-6 and MDA in the serum of rats with myocardial ischemia induced by coronary artery ligation. Under the intervention of Danggui Buxue decoction, the expression of SOD1cat and GPx in oxidative stress-related protein was upregulated, while the expression of MDA5 protein was down-regulated. Conclusion Danggui Buxue decoction has protective effect on myocardial ischemia induced by coronary artery ligation in rats, which may be related to regulating the content of cytokines and protein expression in oxidative stress.
【作者单位】：安徽中医药大学第一附属医院;
【基金】：安徽省自然科学基金(No.1508085SQH228) 安徽中医药大学自然科学基金(No.2015qn023)
【分类号】：R285.5;R-332

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