纳米银用于牙科根管消毒的抗菌作用研究

发布时间：2018-05-31 03:20

本文选题：纳米银 + 根管消毒　；参考：《中国抗生素杂志》2017年03期

【摘要】：目的研究纳米银对引起牙科根管治疗失败的致病菌粪肠球菌、溶血链球菌的抑菌、杀菌和抑制生物被膜形成的作用,为纳米银应用于根管消毒提供实验依据。方法微稀释法测定纳米银及其对照药物对粪肠球菌最小抑菌浓度(MIC)和最小杀菌浓度(MBC)、对溶血链球菌的MIC,评价纳米银的抗菌作用。结晶紫染色法检测粪肠球菌ATCC51299和粪肠球菌ATCC29212,溶血链球菌ATCC6644和溶血链球菌ATCC19615生物被膜形成情况,评价纳米银及对照药物对生物被膜形成的抑制作用。结果纳米银对25株粪肠球菌有较好的抑制作用,MIC_(range)为37.5~75μmol/L,MIC_(50)和MIC_(90)均为75μmol/L;对24株链球菌也有较好的抑制作用,MIC_(range)为18.75~37.5μmol/L,MIC_(50)和MIC_(90)均为37.5μmol/L。纳米银对粪肠球菌ATCC51299和粪肠球菌ATCC29212显示较好的杀菌活性,最小杀菌浓度和最小抑菌浓度比值(MBC/MIC)为2。同时,纳米银对粪肠球菌ATCC51299和粪肠球菌ATCC29212,溶血链球菌ATCC6644和溶血链球菌ATCC19615生物被膜形成有明显抑制作用,2倍MIC浓度的纳米银对生物被膜形成的抑制率为79%~94%。
[Abstract]:Objective to study the effects of silver nanoparticles on bacteriostatic, bactericidal and biofilm formation of Enterococcus faecalis and Streptococcus haemolyticus, which caused the failure of dental root canal treatment, and to provide experimental evidence for the application of nano-silver in root canal disinfection. Methods the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of silver nanoparticles and their control drugs against Enterococcus faecalis were determined by microdilution method. The microbes of streptococcus hemolyticus were tested to evaluate the antibacterial effect of silver nanoparticles. The biofilm formation of streptococcus hemolyticus ATCC6644 and streptococcus hemolyticus ATCC19615 was detected by crystal violet staining to evaluate the inhibitory effect of silver nanoparticles and control drugs on the biofilm formation of Enterococcus faecalis (ATCC51299) and Enterococcus faecalis (ATCC 29212). Results the inhibitory effects of silver nanoparticles on 25 strains of Enterococcus faecalis were better than those on the other 25 strains of Enterococcus faecalis. The results showed that the inhibitory effects of silver nanoparticles on 25 strains of Enterococcus faecalis were 37.5 渭 mol 路L 路L ~ (-1) and 50 渭 mol 路L ~ (-1), respectively, respectively. The inhibitory effects of silver nanoparticles on 25 strains of Enterococcus faecalis were all 75 渭 mol / L 路L ~ (-1), and 18.7537.5 渭 mol 路L ~ (-1 路MIC50) and 90 渭 mol 路L ~ (-1), respectively. Silver nanoparticles showed good bactericidal activity against Enterococcus faecalis ATCC51299 and Enterococcus faecalis ATCC29212. The ratio of minimum bactericidal concentration to minimum inhibitory concentration was 2. At the same time, silver nanoparticles could inhibit the formation of biofilm of Enterococcus faecalis (ATCC51299) and Enterococcus faecalis (ATCC29212), ATCC6644 of Streptococcus haemolyticus and ATCC19615 of Streptococcus haemolyticus. The inhibition rate of silver nanoparticles with twice MIC concentration on the formation of biofilm was 790.94%.
【作者单位】：中国医学科学院北京协和医学院医药生物技术研究所药理研究室抗感染药物研究北京市重点实验室;北京大学口腔医院第二门诊部;
【基金】：国家自然科学基金(No.81200826) 北京科技专项(No.Z141102004414065,No.Z151100000315029)
【分类号】：R781.05

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