细胞外ATP对失神经大鼠腓肠肌细胞中FoXO3a通路的影响

发布时间：2018-05-31 11:02

本文选题：坐骨神经 + 失神经支配　；参考：《山西医科大学》2012年硕士论文

【摘要】：目的:探讨细胞外ATP对失神经大鼠腓肠肌细胞中FoXO3a/MAFbX通路的影响。方法：雌性成年Wistar大鼠54只随机分三组，ATP治疗组和失神经对照组在右侧梨状肌下缘切断坐骨神经，制作失神经动物模型。术后ATP治疗组于右侧腓肠肌内注射ATP 0. 1mg/ d，左侧腓肠肌内注射等量生理盐水；失神经对照组双下肢注射等量的生理盐水，正常对照组（0天）不做任何处理。在术后0，2，7，14，，28天分别处死一组大鼠取其两侧腓肠肌,称肌湿重,计算肌失重比(右侧"左侧)，采用年实时荧光定量PCR和Western Blotting检测大鼠腓肠肌FoxO3a、MAFbx的mRNA和蛋白表达水平以及253位磷酸化的FoxO3a（p-FoxO3aS253）蛋白表达。结果:ATP治疗组肌湿重比高于失神经对照组，差异具有统计学意义（p＜0.01）。ATP治疗组FoxO3amRNA和蛋白表达略低于失神经照组，差异无统计学意义（p＞0.05）。MAFbxmRNA和蛋白表达低于失神经对照组，差异有统计学意义（p＜0.05）。p-FoxO3a蛋白表达量较对照组明显增加，不同时段差异均具有统计学意义（p＜0.05）。结论：细胞外ATP可能通过磷酸化FoxO3a进而抑制MAFbX蛋白的表达来延缓去神经性大鼠肌萎缩。
[Abstract]:Aim: to investigate the effect of extracellular ATP on FoXO3a/MAFbX pathway in gastrocnemius muscle cells of denervated rats. Methods: Fifty-four female adult Wistar rats were randomly divided into three groups: the ATP-treated group and the denervated control group. The sciatic nerve was cut off at the lower edge of the right piriformis muscle to make the denervated animal model. ATP was injected into the right gastrocnemius muscle in the ATP group after operation. 1mg/ d, the left gastrocnemius muscle was injected with the same amount of saline in the left gastrocnemius muscle and the denervated control group was injected with the same amount of saline in the lower extremities of the denervated group, and the normal control group was given 0 days The gastrocnemius muscles were taken from both sides of the gastrocnemius muscle and weighed wet on day 1428 after operation. The muscle weight loss ratio (right) was calculated. The expression of mRNA and protein in the gastrocnemius muscle FoxO3a, MAFbx and phosphorylated FoxO3ap-FoxO3aS253 protein were detected by annual real-time quantitative PCR and Western Blotting. Results the wet weight ratio of muscle in the treatment group was higher than that in the denervated control group, and the difference was statistically significant (P < 0.05). The expression of FoxO3amRNA and protein in the 0.01).ATP group was slightly lower than that in the denervated group. There was no significant difference in the expression of 0.05).MAFbxmRNA and protein between the two groups. The difference was statistically significant (P < 0.05). The expression of 0.05).p-FoxO3a protein was significantly higher than that of the control group. Conclusion: extracellular ATP may delay denervated rat muscle atrophy by phosphorylated FoxO3a and inhibit the expression of MAFbX protein.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R329

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