远志石菖蒲药对对注意缺陷多动障碍模型大鼠夜周期行为学的影响

发布时间：2018-06-01 05:30

本文选题：注意缺陷多动障碍 + 远志　；参考：《中医杂志》2017年20期

【摘要】：目的探讨远志-石菖蒲药对治疗注意缺陷多动障碍(ADHD)的可能作用机制。方法将30只自发性高血压(SHR)大鼠随机分为模型组、阳性对照组、远志-石菖蒲组,每组10只,另设WKY大鼠10只为对照组。远志-石菖蒲组给予远志-石菖蒲混悬液4.76 mg/(kg·d)灌胃;阳性对照组给予利他林混悬液2mg/(kg·d)灌胃;正常组和模型组均以0.5%羧甲基纤维素钠溶液2 ml/(kg·d)灌胃,在大鼠夜周期9:30~10:30、15:30~16:30分两次灌胃,连续给药4周。分别于给药前、给药4周及停药2周时,采用开场实验评价大鼠自发性行为;给药4周采用高架十字迷宫、新物体识别实验评估大鼠冲动行为及学习记忆功能。停药2周时检测前额叶皮质中多巴胺(DA)、多巴胺转运体(DAT)水平。结果给药4周远志-石菖蒲组大鼠在开场实验中的总运动距离及直立次数、进入高架十字迷宫开臂次数百分比和开臂滞留时间百分比均低于模型组(P0.05),而在新物体识别实验对新物体的偏好指数则高于模型组(P0.05);停药2周远志-石菖蒲组大鼠总运动距离及直立次数仍少于模型组,前额叶皮质中DA水平较模型组明显升高(P0.05),而DAT含量与模型组间差异无统计学意义(P0.05)。结论远志-石菖蒲药对可提高ADHD小鼠前额叶皮质中DA含量,可能是其治疗ADHD的作用机制之一。
[Abstract]:Objective to explore the possible mechanism of Radix Polygali-Acorus calamus on ADHD in the treatment of attention deficit hyperactivity disorder (ADHD). Methods Thirty spontaneously hypertensive SHRs were randomly divided into three groups: model group, positive control group, Rhizoma Polygala and Acorus calamus group with 10 rats in each group and 10 WKY rats as control group. The control group was given Ritalin suspension (2mg/(kg d), the normal group and the model group were given 0.5% sodium carboxymethyl cellulose solution for 2 ml/(kg d, and the rats were perfused twice at 9: 30: 30: 3015: 3016: 30 during the night period of 9: 30: 30: 3015: 30: 30: 30, respectively, and the rats in the model group were treated with 0.5% sodium carboxymethyl cellulose solution for 2 ml/(kg d. The drug was given continuously for 4 weeks. The spontaneous behavior of rats was evaluated by open field experiment before administration, 4 weeks before administration and 2 weeks after withdrawal, and by using elevated cross maze at 4 weeks. The impulsive behavior and learning and memory function of rats were evaluated by new object recognition experiment. At 2 weeks after withdrawal, the levels of dopamine cinnamon and dopamine transporter were detected in the prefrontal cortex. Results the total distance of movement and the number of times of standing up in the open field experiment of the rats in the group of Polygala acuminata and Acorus calamus were given for 4 weeks. The percentage of open arm times and open arm retention time in the elevated cross maze was lower than that in the model group, but the preference index of the new object recognition experiment was higher than that of the model group. The total movement distance and the number of upright times were still less than those of the model group. The DA level in the prefrontal cortex was significantly higher than that in the model group (P 0.05), but there was no significant difference between the DAT content and the model group (P 0.05). Conclusion Radix Polygala and Acorus calamus can increase DA content in prefrontal cortex of ADHD mice, which may be one of the mechanisms of its therapeutic effect on ADHD.
【作者单位】：深圳市中医院;深圳市老年医学研究所;
【基金】：国家自然科学基金(81503616) 中国博士后科学基金(2016M590840) 广东省省级科技计划(2014A020221003) 广东省中医药局科研项目(20161218) 深圳市科技计划(JCYJ20160428105935612) 深圳市卫生计生系统科研项目(201505019)
【分类号】：R285.5;R-332

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