米非司酮对子宫内膜异位症模型大鼠炎症因子表达的影响

发布时间：2018-06-02 02:34

本文选题：米非司酮 + 子宫内膜异位症　；参考：《中国药房》2016年10期

【摘要】：目的:研究米非司酮对子宫内膜异位症(Ems)模型大鼠炎症因子表达的影响。方法:取SD大鼠,随机分为正常(生理盐水)组、模型(生理盐水)组和米非司酮低、中、高剂量[0.65、1.30、2.60 mg/(kg·d)]组,每组10只。除正常组外,其余各组大鼠复制Ems模型,各组大鼠ig相应剂量的药物,连续4周。比较各组大鼠治疗前后异位病灶体积,酶联免疫吸附法检测各组大鼠血清中肿瘤坏死因子α(TNF-α)、白细胞介素(IL)-6、IL-8含量,Western blot法分析各组大鼠异位病灶组织中环氧化酶2(COX-2)、前列腺素E2(PGE_2)蛋白表达及核因子κB(NF-κB)p65磷酸化水平,实时定量-聚合酶链反应法检测各组大鼠异位病灶组织中NF-κB p65m RNA表达水平。结果:与正常组比较,模型组大鼠异位病灶体积和血清TNF-α、IL-6、IL-8含量增加,病灶组织中COX-2、PGE_2蛋白和NF-κB p65磷酸化水平及m RNA表达增强(P0.01);与模型组比较,米非司酮各剂量组大鼠异位病灶体积减小,血清TNF-α、IL-6、IL-8含量降低,病灶组织中COX-2、PGE_2和NF-κB p65磷酸化水平及m RNA表达减弱(P0.01),且呈剂量依赖性。结论:米非司酮能减小Ems异位病灶体积,可能与抑制NF-κB信号通路和降低炎症因子表达有关。
[Abstract]:Aim: to study the effect of mifepristone on the expression of inflammatory factors in endometriosis rats. Methods: SD rats were randomly divided into normal saline group, model group and mifepristone group with 10 rats in each group. The Ems model was established in the other groups except the normal group. The rats in each group were given the same dose of drug for 4 weeks. The volume of ectopic focus was compared before and after treatment in each group. Serum TNF- 伪 and IL-8 levels in serum of rats in each group were detected by enzyme-linked immunosorbent assay (Elisa). The expression of cyclooxygenase 2COX-2and prostaglandin E2PGE2) protein and the phosphorylation of NF- 魏 B NF- 魏 B)p65 in ectopic lesions of rats in each group were analyzed by Western blot. The expression of NF- 魏 B p65m RNA in ectopic lesions of rats was detected by real-time quantification-polymerase chain reaction. Results: compared with the normal group, the volume of ectopic focus and the content of serum TNF- 伪 -IL-6 IL-8 in the model group were increased, and the phosphorylation levels of COX-2 PGE2 protein and NF- 魏 B p65 and the expression of m RNA in the lesion tissue were increased (P 0.01), compared with the model group. The volume of ectopic focus and the level of IL-8 in serum TNF- 伪 were decreased in different dose groups of mifepristone. The phosphorylation levels of COX- 2 PGE2 and NF- 魏 B p65 and the expression of m RNA were decreased in a dose-dependent manner. Conclusion: mifepristone can reduce the volume of Ems ectopic focus, which may be related to the inhibition of NF- 魏 B signaling pathway and the decrease of inflammatory factor expression.
【作者单位】：青海省妇女儿童医院妇产科;青海省地方病预防控制所;
【分类号】：R965;R-332

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