基于斑马鱼模型的26种常见骨伤科中药材的毒性筛选

发布时间：2018-06-04 01:27

本文选题：斑马鱼 + 骨伤科中药　；参考：《南京中医药大学学报》2016年05期

【摘要】：目的利用斑马鱼毒性模型评价26种常见伤科中药材的安全性。方法将受精后1d(1dpf)的健康斑马鱼胚胎置于不同浓度药味水煎液中,观察记录给药后1~5d的死亡情况,显微检视3dpf或以后斑马鱼幼鱼的形态,同时设置0.4%DMSO溶媒对照组。用SPSS16.0计算不同药味水煎液在6dpf的鱼半数致死浓度LC_(50)。结果 13种药味(续断、知母、乳香加没药、补骨脂、独活、牛膝、制何首乌、蛇床子、木香、土鳖虫、淫羊藿和五加皮)水煎液致斑马幼鱼脏器形态明显改变,毒性主要表现为卵黄囊肿大、变形、变黑,心包水肿、出血等,且它们的鱼LC_(50)值较低(16.9~689.7μg/mL)。其余13种药味(鹿衔草、千年健、苏木、巴戟天、龟甲、骨碎补、丹参、狗脊、桑寄生、鸡血藤、熟地黄、女贞子、杜仲),未见鱼脏器毒性,且致鱼死亡浓度高(在2 000μg/mL以上),较安全。结论用斑马鱼模型成功的筛选了26种常见骨伤科中药的毒性,为骨伤科中药临床合理应用提供参考。斑马鱼模型具有简单、高效、实时动态的优势,使中药在体毒性规模化筛选成为可能。
[Abstract]:Objective to evaluate the safety of 26 common injured Chinese medicinal materials by zebrafish toxicity model. Methods the healthy zebrafish embryos of 1 day after fertilization were placed in different concentrations of medicinal flavor decoction. The death of juvenile zebrafish was observed and recorded at 1 ~ 5 days after administration. The morphology of juvenile zebrafish (3dpf) or the later zebrafish was examined microscopically, and the control group was set up with 0.4%DMSO solvent at the same time. SPSS16.0 was used to calculate the LD50 of fish with different medicinal flavors in 6dpf. Results 13 kinds of medicinal flavors (Dipsacus, Anemarrhena, frankincense plus myrrh, psoralen, Radix angelicae, Radix Polygoni multiflorum, Cnidium Cnidii, Radix Cynthopsis, hillbilly insect, Epimedium and Cortex Acanthopsis) were significantly changed in the organs of juvenile zebra fish. The main toxic manifestations were yolk sac swelling, deformation, blackening, pericardial edema, hemorrhage, etc. The remaining 13 kinds of medicinal flavors (Herba Deer, Chienjian, Sappa, Morinda officinalis, tortoise shell, shredder, salvia miltiorrhiza, dog ridge, mulberry parasitism, chicken blood rattan, cooked Rehmannia, Ligustrum lucidum, Eucommia ulmoides, no toxicity to fish viscera), It was safe to cause fish death (above 2 000 渭 g/mL). Conclusion the toxicity of 26 kinds of common orthopedic Chinese medicine was successfully screened by zebrafish model, which provides a reference for the rational clinical application of orthopedic Chinese medicine. Zebrafish model has the advantages of simple, efficient and real-time dynamic, which makes it possible to screen in vivo toxicity of traditional Chinese medicine on a large scale.
【作者单位】：南京中医药大学药学院;江苏省中医药研究院国家中医药管理局中药口服释药系统重点研究室江苏;
【基金】：国家自然科学基金(81573833) 江苏省自然科学基金(BK20141507,BK2011866) 江苏省第十批“六大人才高峰”(2013-YY006) 江苏省第四期“333工程”科研资助项目(BRA2014348)
【分类号】：R285.5;R-332

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