Elmo1通过激活Rac促进中性粒细胞ROS的产生

发布时间：2018-06-04 08:08

本文选题：Elmo + 中性粒细胞　；参考：《现代免疫学》2017年04期

【摘要】：本研究探讨Elmo1对PMA刺激的中性粒细胞产生ROS的调控作用。分离WT(Elmo1+/+)和Elmo1-/-小鼠骨髓来源的中性粒细胞后,FACS法检测中性粒细胞的纯度。用PMA刺激中性粒细胞,通过分子探针标记检测ROS的产量,利用亲和沉淀GTP-Rac的方法研究Rac的激活情况,进而探索Elmo1对ROS和Rac活性的影响以及其中的作用机制。结果显示:小鼠中性粒细胞表面标志物Gr-1和CD11b的双阳性率高达90%以上。PMA刺激后,Elmo1-/-小鼠中性粒细胞ROS的产量明显低于WT(P0.05),且Rac1和Rac2的激活程度也显著弱于WT(P0.05)。使用Rac抑制剂后,Elmo1+/+和Elmo1-/-的中性粒细胞ROS产量之间的差异消失了。以上结果揭示了Elmo1通过激活Rac促进中性粒细胞产生ROS。
[Abstract]:The aim of this study was to investigate the effect of Elmo1 on the production of ROS by PMA-stimulated neutrophils. WT(Elmo1 /) and neutrophils from bone marrow of Elmo 1-r-mice were isolated and the purity of neutrophils was detected by FCS. Neutrophils were stimulated by PMA, the yield of ROS was detected by molecular probe labeling, the activation of Rac was studied by affinity precipitation of GTP-Rac, and the effect of Elmo1 on the activities of ROS and Rac and its mechanism were explored. The results showed that the double positive rates of Gr-1 and CD11b on the surface of neutrophils in mice were as high as 90%. PMA-stimulated neutrophil ROS production was significantly lower than that of WTP 0.05, and the activation of Rac1 and Rac2 was significantly weaker than that of WTP 0.05. The difference in ROS production between Elmo1 / and Elmo1 /-neutrophils disappeared after the use of Rac inhibitor. These results suggest that Elmo1 stimulates neutrophil production by activating Rac.
【作者单位】：上海交通大学医学院上海市免疫学研究所;
【基金】：国家自然科学基金(31571430;31671234) 国家重点基础研究发展计划(2014CB541804) 上海市卫生和计划生育委员会项目(201440300) 上海市自然科学基金(15ZR1436300) 上海市教育委员会科研创新项目(15ZZ053) 上海市卫生局青年项目(20164Y0149)
【分类号】：R392

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