维生素D3对自然流产模型小鼠胚胎丢失率及外周血免疫细胞的影响

发布时间：2018-06-04 20:02

本文选题：维生素D + 自然流产　；参考：《解剖学报》2017年05期

【摘要】：目的探讨维生素D3(VitD_3)对自然流产模型小鼠胚胎丢失率及外周血免疫细胞的影响。方法采用CBA/J×BALB/c建立正常妊娠小鼠模型,采用CBA/J×DBA/2建立自然流产小鼠模型,实验动物分5组:正常组、自然流产组、VitD_3低剂量组、中剂量组和高剂量组,每组15只。VitD_3各组自妊娠第1天开始腹腔注射VitD_3溶液,单次VitD_3给药剂量分别为1μg、4μg、16μg。记录和观察各组小鼠胚胎丢失情况,检测各组血清调节性T细胞(Treg)细胞因子:白细胞介素-10(IL-10),辅助性T细胞17(Th17)细胞因子:白细胞介素-17A(IL-17A)以及各组外周血Treg、Th17。结果与正常组相比,自然流产组胚胎丢失率显著升高(χ~2=16.045,P0.05);与自然流产组比较,VitD_3低剂量组、中剂量组和高剂量组胚胎丢失率显著降低(χ~2=5.881、13.704、15.663,P0.05)。与正常组相比,自然流产组Treg、IL-10、Treg/Th17、IL-10/IL-17A显著降低,Th17、IL-17A显著升高,差异均有统计学意义(P0.05);与自然流产组比较,VitD_3低剂量组、中剂量组和高剂量组Treg、IL-10、Treg/Th17、IL-10/IL-17A显著升高,Th17、IL-17A显著降低,差异均有统计学意义(P0.05)。结论 VitD_3能够降低自然流产模型小鼠胚胎丢失率,可能与调节Treg/Th17平衡有关。
[Abstract]:Objective to investigate the effect of Vitamin D _ (3) Vitamin D _ (3) on embryo loss and peripheral blood immune cells in spontaneous abortion mice. Methods normal pregnant mice were established by CBA/J 脳 BALB/c and spontaneous abortion by CBA/J 脳 DBA/2. The experimental animals were divided into 5 groups: normal group, spontaneous abortion group, low dose group, middle dose group and high dose group. VitD_3 solution was injected intraperitoneally from the first day of gestation to 15 rats in each group. The dose of VitD_3 was 1 渭 g / g 4 渭 g / g and 16 渭 g / g respectively. The embryo loss in each group was recorded and observed, and the cytokines of serum regulatory T cell (T cell), interleukin-10 (IL-10), helper T cell (17-Th17), interleukin-17A-1 (IL-17A) and peripheral blood Treg-Th17 (Th17) were detected in each group. Results compared with the normal group, the embryo loss rate in the spontaneous abortion group was significantly higher (蠂 ~ 2 / 216.045 / P _ (0.05), and the embryo loss rate in the medium and high dose groups was significantly lower than that in the low dose group (蠂 ~ 25.881) and the high dose group (蠂 ~ 2 / 25.881 ~ 13.704 ~ 15.663P0.05). Compared with the normal group, the Treg-IL-10 / Th17 / IL-17A level in the spontaneous abortion group was significantly lower than that in the control group (P 0.05), and the TregIL-10 Treg-10 Treg-10 / 10 / IL-17A in the low dose group, the middle dose group and the high dose group were significantly higher than that in the normal group, and the difference was statistically significant (P 0.05). Conclusion VitD_3 can reduce the rate of embryo loss in spontaneous abortion mice, which may be related to the regulation of Treg/Th17 balance.
【作者单位】：华中科技大学同济医学院附属武汉儿童医院(武汉市妇幼保健院)妇产科;武汉市中心医院妇产科;华中科技大学同济医学院附属武汉儿童医院(武汉市妇幼保健院)病理科;
【分类号】：R-332;R714.21

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