大鼠腰椎“椎骨错缝”模型痛阈变化研究

发布时间：2018-06-07 11:38

本文选题：椎骨错缝 + 痛阈　；参考：《中华中医药学刊》2017年11期

【摘要】：目的:观察腰椎"椎骨错缝"模型大鼠痛阈变化。方法:采用椎体外部连接固定系统(ELFS)诱导"椎骨错缝"大鼠模型。将160只350~450 g雄性SD大鼠随机分为正常对照组、假手术组、模型对照组和实验组。实验组通过外科手术将ELFS的棘突附着部件(SAU)与大鼠L4、L5和L6棘突相连接,再使用ELFS的外部连接部件将SAU连接起来;模型对照组仅植入SAU,不安装外部连接部件;假手术组仅进行手术干预,不植入任何部件;正常对照组不进行任何干预。再将各组按固定时间分为1、2、4、8和12周5个亚组。对每组中8只大鼠进行机械痛阈和热痛阈检测。结果:实验组的机械痛阈在4周及8周时与对照组出现统计学差异(P0.05);实验组各时间点间机械痛阈随时间延长呈逐渐下降的趋势。实验组的热痛阈在2周时间点时与对照组出现统计学差异(P0.05),这种差异一直持续到8周;实验组各时间点间热痛阈值呈逐渐下降的趋势,4周时热痛阈和1、2周时间点相比出现显著差异(P0.05),并持续到8周。结论:"椎骨错缝"模型大鼠现伴随着生物力学的改变,其行为学也发生着相似的变化。
[Abstract]:Objective: to observe the change of pain threshold of lumbar vertebrae dislocation suture model. Methods: the rat model of dislocation suture of vertebral bone was induced by the external vertebral junction fixation system (ELFS). 160 male SD rats were randomly divided into normal control group, sham operation group, model control group and experimental group. In the experimental group, the spinous process attachment of ELFS was connected with the spinous process of L4, L5 and L6 by surgical operation, and then the SAU was connected with the external connective part of ELFS, and the model control group was only implanted with sau, and no external connectors were installed. The sham-operation group only performed surgical intervention and no parts were implanted, while the normal control group did not intervene. Each group was divided into 5 subgroups according to the fixed time. Mechanical pain threshold and thermal pain threshold were measured in 8 rats in each group. Results: the mechanical pain threshold of the experimental group was significantly different from that of the control group at 4 and 8 weeks, and the mechanical pain threshold of the experimental group decreased gradually with the prolongation of time. The thermal pain threshold of the experimental group was significantly different from that of the control group at the point of 2 weeks, and the difference lasted until 8 weeks. The thermal pain threshold of the experimental group decreased gradually at each time point. There was a significant difference between the thermal pain threshold at 4 weeks and that at 1 and 2 weeks (P 0.05) and lasted until 8 weeks. Conclusion: the changes of biomechanics and behavior of the rat model of vertebrae dislocation occurred.
【作者单位】：上海中医药大学附属岳阳中西结合医院;南京中医药大学第二临床医学院;上海市中医药研究院推拿研究所;上海市中医药研究院骨伤科研究所;
【基金】：国家自然科学基金面上项目(81273869,81403496)
【分类号】：R244.1;R-332

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