CEA迷你基因串联体疫苗的体内抗肿瘤活性观察

发布时间：2018-06-08 05:17

本文选题：癌胚抗原 + 迷你基因　；参考：《中国免疫学杂志》2017年01期

【摘要】：目的:观察已构建的含有CEA_(625-667)基因单倍体疫苗pc DNA-CEA_(625-667)及三串联体的DNA疫苗pc DNA-triCEA_(625-667)对荷瘤小鼠体内肿瘤的抑制情况及小鼠存活时间的改变。方法:建立小鼠肝细胞癌实验动物模型,应用单倍体疫苗pc DNA-CEA_(625-667)及三串联体DNA疫苗pc DNA-tri CEA_(625-667)免疫小鼠,以生理盐水为对照组,观察各实验组小鼠皮下肿瘤生长情况,记录皮下肿瘤生长曲线,观察疫苗对荷瘤小鼠肿瘤生长速度的影响以及疫苗对荷瘤小鼠生存时间的影响。结果:与生理盐水对照组相比,两种疫苗均能明显抑制CEA阳性荷瘤小鼠的肿瘤体积以及生长速度(P0.01),其中,pc DNA-tri CEA_(625-667)疫苗组的抑制作用明显优于pc DNA-CEA_(625-667)疫苗组(P0.01),而二者均不能抑制CEA阴性荷瘤小鼠的肿瘤生长。pc DNACEA_(625-667)疫苗组平均生存时间为(48.50±6.73)d,与生理盐水对照组(39.00±6.64)d相比有显著差异(P0.01);pc DNA-triCEA_(625-667)疫苗组生存时间(48.50±6.73)d明显高于生理盐水对照组和pc DNA-CEA_(625-667)疫苗组(P0.01)。两组疫苗均不能延长CEA阴性荷瘤小鼠的生存时间。结论:无论是单倍体还是三串联体的DNA疫苗,均能够明显抑制CEA阳性荷瘤小鼠的肿瘤生长速度(P0.01)及明显延长其生存时间(P0.01),而对CEA阴性荷瘤小鼠则无治疗作用。
[Abstract]:Aim: to observe the inhibitory effect of pcDNA-CEA7) and triplex DNA vaccine pcDNA-triCEA (625-667) on tumor and the survival time of mice. Methods: mice were immunized with haploid vaccine pcDNA-CEA (625-667) and triple tandem DNA vaccine (pcDNA-tri CEA-1) 625-667). Normal saline was used as control group to observe the growth of subcutaneous tumor in mice. The growth curve of subcutaneous tumor was recorded to observe the effect of vaccine on tumor growth rate and survival time of tumor bearing mice. Results: compared with normal saline control group, Both vaccines could significantly inhibit the tumor volume and growth rate of CEA positive tumor-bearing mice (P0.01). The inhibitory effect of the pc-DNA-tri CEAAJI (625-667) vaccine group was significantly better than that of the PC DNA-CEAMA-625-667) vaccine group, but neither of them could inhibit the tumor of CEA negative tumor-bearing mice. The average survival time of the vaccine group was 48.50 卤6.73 days, which was significantly higher than that of the saline control group (39.00 卤6.64 days). The survival time of the vaccine group was 48.50 卤6.73 days longer than that of the normal saline control group and the pcDNA-CEAstin 625-667) vaccine group. Neither vaccine could prolong the survival time of CEA negative tumor-bearing mice. Conclusion: both haploid and triplex DNA vaccines can significantly inhibit the tumor growth rate and prolong the survival time of CEA positive tumor-bearing mice (P0.01), but have no therapeutic effect on CEA negative tumor-bearing mice.
【作者单位】：吉林省人民医院医学诊治实验中心;吉林大学第一医院呼吸内科;
【基金】：吉林省科技厅国际合作项目(20140414043GH);吉林省科技厅中青年科技创新领军人才及团队项目(20140519018JH);吉林省科技厅重点实验室项目(20122113)资助吉林省人社厅省人才开发资金(2016年度)
【分类号】：R392-33

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