淫羊藿苷对阿尔茨海默病大鼠模型金属蛋白酶及紧密连接蛋白ZO-1表达的影响

发布时间：2018-06-08 15:57

本文选题：阿尔茨海默病 + 淫羊藿苷　；参考：《中风与神经疾病杂志》2017年10期

【摘要】：目的探讨淫羊藿苷对阿尔茨海默病(Alzheimer's disease,AD)大鼠学习记忆、海马区基质金属蛋白酶-9(MMP-9)、MMP-2及血脑屏障紧密连接蛋白ZO-1表达的影响。方法采用Aβ1-42海马注射建立AD大鼠模型,使用淫羊藿苷进行干预。以Morris水迷宫检测大鼠的空间学习记忆能力,采用Western-blot法分析紧密连接蛋白ZO-1的表达,用免疫组织化学法检测大鼠海马区MMP-9、MMP-2表达。结果与对照组相比,AD模型组大鼠学习记忆能力明显减退,ZO-1表达明显减少(P0.05),MMP-9、MMP-2蛋白明显增加;淫羊藿苷干预后,大鼠学习记忆能力均明显提高,海马区MMP-9/MMP-2表达减少(P0.05),ZO-1表达明显增加(P0.05),以淫羊藿苷高剂量干予后明显。结论淫羊藿苷能通过抑制MMP-9/MMP-2表达,减轻血脑屏障破坏,从而改善AD症状。
[Abstract]:Objective to investigate the effects of icariin on learning and memory, matrix metalloproteinase-9 (MMP-9) -9 (MMP-2) and tight junction protein ZO-1 (ZO-1) in rats with Alzheimer's disease (AD). Methods A 尾 1-42 rat model of AD was established by hippocampal injection and treated with icariin. The spatial learning and memory ability of rats was measured by Morris water maze, the expression of tight junction protein ZO-1 was analyzed by Western-blot, and the expression of MMP-9 and MMP-2 in hippocampus was detected by immunohistochemistry. Results compared with the control group, the learning and memory ability of rats in AD model group decreased significantly, the expression of ZO-1 decreased significantly, and the expression of MMP-9 MMP-2 protein increased significantly, and the ability of learning and memory increased significantly after the intervention of icariin. The expression of MMP-9 / MMP-2 in hippocampus decreased the expression of P0.05 and ZO-1 increased significantly, especially after high dose of icariin. Conclusion Icariin can ameliorate the symptoms of AD by inhibiting the expression of MMP-9 / MMP-2 and alleviating the damage of blood-brain barrier.
【作者单位】：广西医科大学第四附属医院神经内科;广西医科大学第一附属医院神经内科;
【基金】：国家自然科学基金(81460813) 广西区计划项目青年基金(Z2015145)
【分类号】：R285.5;R-332

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