大鼠大脑皮质星形胶质细胞α2A型肾上腺受体的表达研究

发布时间：2018-06-09 15:53

  本文选题：αA肾上腺素能受体 + 星形胶质细胞　； 参考：《医学研究杂志》2016年09期

【摘要】：目的验证大鼠大脑皮质星形胶质细胞中的α2A肾上腺素能受体(α2A-AR)的表达情况,并与α2B肾上腺素能受体(α2B-AR)、α2C肾上腺素能受体(α2C-AR)相比较。方法获取目的基因α2A-AR、α2B-AR、α2C-AR的RNA反转录,与内参基因β-actin一起进行实时荧光定量PCR。重复6组,所得数据进行统计学分析并运用2-△△Ct的方法进行计算。结果大鼠大脑皮质星形胶质细胞中确实表达了α2A-AR,并且α2A-AR的相对表达量是α2B-AR的24.53倍,是α2C-AR的27.98倍。结论α2A-AR可能为大鼠大脑皮质星形胶质细胞接受儿茶酚胺类神经递质调控的主要受体。
[Abstract]:Objective to investigate the expression of 伪 2A adrenergic receptor (伪 2A-ARR) in rat cerebral cortex astrocytes and compare it with 伪 2B adrenoceptor (伪 2B-ARA, 伪 2C adrenoceptor (伪 2C-ARR). Methods RNA reverse transcriptions of target genes 伪 2A-AR, 伪 2B-ARand 伪 2C-AR were obtained, and real-time quantitative PCRs were performed with 尾 -actin. Repeat 6 groups, the data were statistically analyzed and calculated by 2-Ct method. Results 伪 2A-AR was expressed in astrocytes of rat cerebral cortex, and the relative expression of 伪 2A-AR was 24.53 times of 伪 2B-AR and 27.98 times of 伪 2C-AR. Conclusion 伪 2A-AR may be the main receptor of catecholamine neurotransmitters in astrocytes of rat cerebral cortex.
【作者单位】： 遵义医学院、贵州省麻醉与器官保护基础研究重点实验室;
【基金】：国家自然科学基金资助项目(81460219)
【分类号】：R338
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本文编号：2000291