温阳振衰颗粒对慢性心衰模型大鼠心肌中MEF2表达的影响

发布时间：2018-06-12 00:11

本文选题：温阳振衰颗粒 + 慢性心衰　；参考：《中国药房》2017年34期

【摘要】：目的:研究温阳振衰颗粒对慢性心衰(CHF)模型大鼠心肌中肌细胞增强子(MEF2)表达的影响。方法:将60只大鼠随机抽取10只作为正常对照组(生理盐水),其余50只大鼠以阿霉素建立CHF模型后随机分为模型对照组(生理盐水)、阳性对照组[依那普利1.8 mg/(kg·d)]和温阳振衰颗粒低、中、高剂量组[0.72、1.44、2.88 g/(kg·d)],每天ig给药2次,连续给药4周。给药结束后,检测各组大鼠心功能指标[左心室射血分数(LVEF)、左心室短轴缩短率(LVFS)、左心室收缩末期内径(LVSD)和左心室舒张末期内径(LVDD)],检测各组大鼠心肌中MEF2和磷酸化MEF2(p-MEF2)mRNA及其蛋白的表达。结果:与正常对照组比较,模型对照组和各给药组大鼠LVEF、LVFS明显降低(P0.05),LVSD、LVDD明显升高(P0.05);心肌中p-MEF2蛋白表达明显下调(P0.05),MEF2 mRNA及蛋白表达差异无统计学意义(P0.05)。与模型对照组比较,各给药组大鼠心功能指标均明显改善(P0.05);心肌中p-MEF2蛋白表达均明显上调(P0.05),MEF2 mRNA及蛋白表达差异均无统计学意义(P0.05)。结论:温阳振衰颗粒能拮抗阿霉素诱导的MEF2蛋白磷酸化的下调作用,这可能是其治疗CHF的机制之一。
[Abstract]:Aim: to study the effect of Wenyang Zhenshuai granule (WJG) on the expression of myocyte enhancer MEF2 in rat model of chronic heart failure (CHF). Methods: ten rats were randomly selected as normal control group (normal saline group), and the other 50 rats were randomly divided into model control group (normal saline group), positive control group [enalapril 1.8 mg/(kg d] after doxorubicin was used to establish CHF model. And Wenyang Zhenshuai granules are low, The middle and high dose group [0.72V 1.44U 2.88 g/(kg d] was given intravenously twice a day for 4 weeks. After the administration, Left ventricular ejection fraction (LVVEFN), left ventricular shortening rate (LVFSN), left ventricular end-systolic diameter (LVSD) and left ventricular end-diastolic diameter (LVDDD) were measured, and the expression of MEF2 and phosphorylated MEF2Op-MEF2 mRNA and protein were detected. Results: compared with the normal control group, LVEFV LVFS in the model control group and each administration group was significantly lower than that in the control group, and the LVDD of LVSD in the LVSD group was significantly higher than that in the normal control group, while the expression of p-MEF2 protein in the myocardium was significantly lower than that in the control group, and there was no significant difference in the expression of P0.05 and MEF2 mRNA and protein between the two groups. Compared with the model control group, the cardiac function indexes of rats in each administration group were significantly improved, and the expression of p-MEF2 protein was significantly up-regulated in myocardium. There was no significant difference in mRNA and protein expression between the two groups. Conclusion: Wenyang Zhenshuai granule can antagonize the down-regulation of MEF2 protein phosphorylation induced by adriamycin, which may be one of the mechanisms of its treatment of CHF.
【作者单位】：湖南中医药大学第一附属医院科研科;贵阳中医学院基础医学院;湖南中医药大学第一附属医院中心重症加强护理病房;湖南中医药大学第一附属医院;
【基金】：国家自然科学基金资助项目(No.81173213、81704-061) 湖南省教育厅资助项目(No.15C1047) 2016年度湖南省中医药科研计划项目(No.201651)
【分类号】：R285.5;R-332

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