P-选择素缺失对小鼠生理特征的影响

发布时间：2018-06-12 07:25

本文选题：P-选择素 + P-选择素敲除小鼠　；参考：《中国实验动物学报》2017年01期

【摘要】：目的探讨P-选择素基因(P-selectin)缺失对小鼠生理特征的影响。方法 P-选择素敲除(PKO)小鼠和C57小鼠饲养于SPF级环境中,通过基因鉴定确定其为纯和PKO小鼠。观察PKO小鼠的活动状态、繁育状况,检测血常规,确定P-选择素敲除后对小鼠一般情况的影响。分别于6周和18周处死小鼠,通过伊红苏木素(HE)染色,观察P-选择素敲除后对小鼠各个脏器组织结构的影响。结果 PKO小鼠与正常C57小鼠的繁育状况相似,血常规无异常,HE染色发现PKO小鼠肝、脾、肺、肾组织结构均较正常。结论 P-选择素的缺失不会对小鼠生殖繁育、血液造成影响,对重要的组织脏器也未见明显影响,为以后进一步研究P-选择素在疾病中的作用提供了动物模型研究理论基础。
[Abstract]:Objective to investigate the effect of the deletion of P- selectin gene (P-selectin) on the physiological characteristics of mice. Methods P- selectin knockout (PKO) mice and C57 mice were bred in the SPF environment, and were identified as pure and PKO mice by gene identification. The activity status, breeding status, blood routine test of PKO mice were observed and the mice were determined to be knocked out of the P- selectin. The effect of the situation. The mice were killed at 6 and 18 weeks respectively. The effects of P- selectin on the tissue structure of various organs of mice were observed by HE staining. Results the breeding status of PKO mice was similar to that of normal C57 mice, and there was no abnormal blood routine. HE staining found that the structure of the liver, spleen, lung and kidney of PKO mice was more normal. Conclusion P- is more normal. The deletion of selectin would not affect the reproduction and blood of mice, and had no obvious influence on the important organs and organs. It provided the theoretical basis for the further study of the role of P- selectin in the disease.
【作者单位】：广东药科大学基础学院血管生物学研究所;
【基金】：国家自然科学基金面上项目(31471290) 广东省科技计划项目(51343072)
【分类号】：R-332

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