去泛素化酶nonstop调控果蝇眼睛锥细胞发育的研究

发布时间：2018-06-12 11:31

本文选题：去泛素化酶 + nonstop　；参考：《中国细胞生物学学报》2016年08期

【摘要】：去泛素化酶是一类参与蛋白质稳定性调控的酶,广泛存在于真核生物细胞中,主要功能是水解底物蛋白质上的泛素链。去泛素化酶参与多种重要的生命活动,包括细胞周期调控、细胞信号转导、蛋白质降解、DNA修复及基因表达等,其异常表达与多种疾病的发生密切相关。该文以果蝇为研究对象,探讨去泛素化酶nonstop(not)对果蝇眼睛锥细胞发育分化的调控作用。结果表明,在not功能缺失的果蝇三龄幼虫眼睛成虫盘中,Cut蛋白质水平发生了显著下调;同时,在果蝇蛹期眼睛的分析结果显示,not功能缺失可导致锥细胞和初级色素细胞的分化异常。进一步研究发现,not功能缺失还可导致R1/R6光感受器细胞分化异常及转录因子D-Pax2表达水平异常。然而,not功能缺失并不影响转录因子Lozenge(Lz)的蛋白质水平及EGFR(epidermal growth factor receptor)和Notch信号通路的活性。综上所述,该文结果表明,not可能通过调控D-Pax2的表达水平与R1/R6光感受器细胞的分化影响锥细胞和初级色素细胞的分化。
[Abstract]:De-ubiquitin is a class of enzymes involved in the regulation of protein stability. It widely exists in eukaryotic cells and its main function is to hydrolyze the ubiquitin chain on the substrate protein. Diubiquitin is involved in many important life activities, including cell cycle regulation, cell signal transduction, protein degradation DNA repair and gene expression, and its abnormal expression is closely related to the occurrence of many diseases. The aim of this study was to investigate the effects of nonstopnotase on the development and differentiation of eye cone cells in Drosophila melanogaster (Drosophila melanogaster). The results showed that the level of cut protein was significantly down-regulated in the adult disc of the third instar larva of Drosophila melanogaster, which was deficient in not function, at the same time, The results of eye analysis during the pupa stage of Drosophila melanogaster showed that the absence of not can lead to abnormal differentiation of cone cells and primary pigment cells. Furthermore, it was found that the loss of function of R1 / R6 photoreceptor cells also resulted in abnormal differentiation of R1 / R6 photoreceptor cells and abnormal expression of transcription factor D-Pax2. However, the loss of function did not affect the protein level of transcription factor Lozengege Lz. and the activity of EGFR epidermal growth factor receptor) and Notch signaling pathway. In conclusion, these results suggest that the differentiation of cone cells and primary pigment cells may be affected by regulating the expression of D-Pax2 and the differentiation of R1 / R6 photoreceptor cells.
【作者单位】：温州医科大学眼视光学院;
【基金】：温州医科大学人才启动项目(批准号:QTJ08012);温州医科大学附属眼视光医院人才启动项目(批准号:KYQD141103)资助的课题~~
【分类号】：Q78;R339.14

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