迟钝爱德华菌对大鲵的感染和死疫苗初次免疫保护作用

发布时间：2018-06-12 15:18

本文选题：大鲵 + 迟钝爱德华菌　；参考：《遵义医学院》2011年硕士论文

【摘要】：目的：研究迟钝爱德华菌对大鲵的致病作用；制备死疫苗并探讨其保护作用,为大鲵的迟钝爱德华菌病防治奠定基础。方法：制备甲醛灭活的迟钝爱德华菌死疫苗,经小鼠做毒性试验无不良反应。将15尾体重25g至300g的健康大鲵随机分为对照组、感染组、免疫组(107个/ml、108个/ml、109个/ml),每组3尾。对照组和感染组第1周肌注0.1ml生理盐水。免疫组第1周肌注0.1ml浓度分别为107个/ml、108个/ml、109个/ml死疫苗。第7周,对照组肌注O.1ml生理盐水外,感染组和免疫组肌注0.1ml 107 cfu/ml迟钝爱德华活菌。48小时,取组织冰冻切片,以兔抗迟钝爱德华菌血清为一抗免疫荧光法检测感染后组织器官中细菌分布。采用TUNEL法检测各组大鲵肝、脾组织细胞凋亡。结果：与对照组比较活菌攻击后感染组、免疫组大鲵均出现活动减少、离群独居现象,免疫组恢复较快。攻击后48小时组织免疫荧光技术发现,感染组,免疫肝、脾组织可出现颗粒状、块状荧光物质,免疫组中可见荧光较感染组少。进行凋亡率测定的肝、脾组织,感染组均较对照组高,差异均有统计学意义(P0.05)；各免疫组与对照组比较、各免疫组与感染组比较、各免疫组间比较差异均无统计学意义(P0.05)。结论：1.肌注0.1ml 107cfu/ml迟钝爱德华活菌能引起大鲵的感染,出现活动减少、离群独居现象,且能在肝、脾组织中检测到细菌。2.细胞凋亡可能是大鲵迟钝爱德华菌感染的致病机理之一。3.死疫苗初次免疫效果欠佳,仍需一次以上注射免疫的研究。
[Abstract]:Objective: to study the pathogenicity of Edwardian dullus on giant salamander, prepare the dead vaccine and discuss its protective effect, so as to lay a foundation for the prevention and treatment of Edwardian dullness of giant salamander. Methods: a formalin inactivated vaccine against Edwardsiella dullus was prepared. There was no adverse reaction after toxicity test in mice. Fifteen healthy salamanders weighing 25g to 300g were randomly divided into control group, infection group and immunized group (107, 108, 109, 3 in each group). The control group and the infected group were intramuscularly injected with 0.1ml saline at week 1. In the immunized group, the intramuscular dose of 0.1ml was 107 / ml, 108 / ml, 109 / ml, respectively. At week 7, the control group was intramuscularly injected with 0.1 ml of normal saline, the infected group and the immune group were injected intramuscularly with 0.1ml 107 cfu/ml slow Edward's live bacteria for 48 hours, and the frozen tissue sections were taken. The distribution of bacteria in tissues and organs after infection was detected by immunofluorescence method using rabbit anti-Edwardiella dullerum serum as an anti-immunofluorescence assay. Tunel method was used to detect the apoptosis of liver and spleen cells in each group. Results: compared with the control group, the immunized giant salamander decreased its activity and lived alone, and the immune group recovered faster. 48 hours after attack, it was found that there were granular and massive fluorescent substances in the infected group, immune liver and spleen tissue, and the visible fluorescence in the immunized group was less than that in the infected group. The rate of apoptosis in liver, spleen and infection group was higher than that in control group, the difference was statistically significant (P 0.05), and there was no significant difference between each immunized group and control group, and there was no significant difference between each immune group and infection group. Conclusion 1. The infection of giant salamander could be caused by injecting 0.1ml 107cfu/ml insensitive Edwardian live strain intramuscularly, the activity was decreased, and the isolated bacteria could be detected in liver and spleen tissue. Apoptosis may be one of the pathogenetic mechanisms of Edwardsiella davidianus infection. The primary immunization effect of the dead vaccine is not good enough, and more than one injection immunization is needed.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R392.1

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