退银汤对银屑病样小鼠模型NE、trappin-2、P-cad表达水平的影响

发布时间：2018-06-15 14:41

  本文选题：银屑病 + 退银汤　； 参考：《中国医院药学杂志》2017年01期

【摘要】：目的:观察退银汤对咪喹莫特诱导的银屑病样小鼠模型中皮损组织和血清中性粒细胞蛋白酶(NE)、trappin-2、胎盘型钙黏蛋白(P-cad)等蛋白表达水平的干预作用,探讨退银汤治疗银屑病的作用机制。方法:使用5%咪喹莫特乳膏外涂小鼠背部脱毛区,制备银屑病样小鼠模型;72只小鼠随机分为正常对照组,模型对照组,退银汤低、中、高剂量组,消银颗粒组,每组12只,按照既定实验药量给各用药组灌胃给药,qd,连续14 d,正常对照组和模型对照组给予等容量的生理盐水;采用双抗体酶联免疫吸附法检测模型小鼠皮损组织及血清中NE、trappin-2、P-cad的含量。结果:模型组小鼠皮损组织及血清中NE、trappin-2、P-cad的含量均明显高于正常对照组(P0.01);退银汤各剂量组能明显降低模型小鼠NE、trappin-2、P-cad的表达水平,与模型对照组比较均有显著性差异(P0.05,P0.01);退银汤中、大剂量组降低更为明显,与小剂量组比较均有显著性差异(P0.05,P0.01),并呈现良好的量效依赖关系;退银汤大剂量组皮损组织中的上述蛋白水平已接近正常对照组(P0.05)。结论:退银汤能显著降低咪喹莫特诱导的银屑病样小鼠模型NE、trappin-2、P-cad等蛋白的表达水平,通过降低上述蛋白的表达水平来发挥调节免疫应答功能,可能是退银汤治疗银屑病的作用机理之一。
[Abstract]:Objective: to observe the effect of Tuiyin decoction (TYD) on the expression level of neutrophil proteinase (neutropenase), placental cadherin (Cam) and other proteins in psoriatic mice model induced by Imiquimote, and to observe the effect of TYT on the expression of protein in skin lesions and serum neutrophil protease. To explore the mechanism of Tuiyin decoction in the treatment of psoriasis. Methods: Seventy-two mice with psoriasis were randomly divided into normal control group, model control group, low, medium, high dose group and Xiaoyin granule group with 12 rats in each group. The normal saline was given to the normal control group and the model control group for 14 days, and the content of netrappin-2 P-cad in the skin lesions and serum of the model mice was detected by double antibody enzyme-linked immunosorbent assay (Elisa). Results: the content of netrappin-2P-cad in skin lesion and serum in model group was significantly higher than that in normal control group (P 0.01), and the expression level of NEtrappin-2 P-cad in model mice was significantly decreased in each dose group, which was significantly different from that in model control group (P 0.05P0.01), and in Tuiyin decoction (TYD), the expression of NEtrappin-2P-cad in model mice was significantly lower than that in model control group. The decrease was more obvious in the large dose group than in the low dose group, there was significant difference between the two groups, and there was a good dose-effect relationship between the two groups, and the protein level in the skin lesions of the large dose group was close to that of the normal control group (P 0.05). Conclusion: TYT can significantly reduce the expression level of netrappin-2pcad and other proteins in psoriatic mice induced by Imiquimod, and regulate the immune response by decreasing the expression level of the above proteins. It may be one of the mechanisms of TYT in treating psoriasis.
【作者单位】： 南通市皮肤病性病防治所;
【基金】：南通市指令性社会发展科技计划项目(编号:HS2011031)
【分类号】：R275.9;R-332
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本文编号：2022394