生物医学领域中超连续激光光谱技术的研究进展

发布时间：2018-06-16 05:20

本文选题：超连续谱 + 激光光谱　；参考：《光谱学与光谱分析》2017年02期

【摘要】：超连续谱激光指的是当泵浦激光穿过特殊光波导时,一系列的非线性效应引起入射激光束的光谱展宽,从而输出宽光谱激光束。随着超快激光和光子晶体光纤技术的发展,利用超短脉冲在光子晶体光纤中的传播链产生相干的且亮度高的超连续谱激光成为了一种理想的白光源。自从超连续谱激光源投入应用以来,其应用领域越来越广。尤其在生物医学的细胞、血液等样品分析当中,荧光光谱学、流式细胞仪、共焦显微、光学相干层析等技术都是强有力的分析工具,在采用这些先进技术的科学仪器中,超连续谱激光源成为了一种主要光学部件。首先对超连续谱激光源的国际研究进展作了详细介绍,然后对超连续激光光谱技术在显微成像、流式细胞仪、荧光寿命成像显微、荧光共振能量转移、光学相干层析、共焦显微生物医学分析等生物医学领域中的发展及应用作了综合阐述。对超连续激光光谱技术在非接触式血液制品鉴别的需求、方案及研究进展进行了重点论述,包括覆盖400~2 000nm光谱范围的光纤化轻型超连续谱激光光源研究;采用超连续谱激光光谱方法探索不同物种血液的种属特征;根据大数据的血液样品光谱特征元数据库分析建立数学模型,利用数学模型实现对血液样品种属光谱学判定;血液鉴别光谱分析便携式整机系统研发等。对超连续激光光谱技术在生物医学领域的应用前景作了展望。
[Abstract]:When the pump laser passes through a special optical waveguide, a series of nonlinear effects result in the spectral broadening of the incident laser beam and the output of the wide-spectrum laser beam. With the development of ultra-fast laser and photonic crystal fiber (PCF) technology, coherent and high-brightness supercontinuum laser using ultrashort pulse in photonic crystal fiber has become an ideal white light source. Since the superCW laser source has been put into use, its application field is more and more extensive. Especially in biomedical cell, blood and other sample analysis, fluorescence spectroscopy, flow cytometry, confocal microscopy, optical coherence chromatography and other techniques are powerful analytical tools. The supercontinuum laser source has become one of the main optical components. In this paper, the international research progress of supercontinuum laser sources is introduced in detail, and then the techniques of supercontinuum laser spectroscopy in microscopic imaging, flow cytometry, fluorescence lifetime imaging microscopy, fluorescence resonance energy transfer, optical coherence tomography are introduced in detail. The development and application of confocal microbiomedical analysis and other biomedical fields are reviewed. The requirement, scheme and research progress of supercontinuous laser spectroscopy in non-contact blood products identification are discussed in detail, including the study of optical fiber light supercontinuum laser light source covering 400 ~ 2 000nm spectrum range. The blood species characteristics of different species were explored by using supercontinuum laser spectroscopy, and the mathematical model was established according to the spectral characteristic meta-database of big data's blood samples, and the spectral identification of blood samples was realized by using the mathematical model. Research and Development of Portable whole Machine system for Blood Identification Spectroscopy. The prospect of the application of supercontinuous laser spectroscopy in biomedicine is also discussed.
【作者单位】：中国科学院空间主动光电技术重点实验室;
【基金】：国家高技术研究发展计划863项目(2015AA021103) 国家自然科学基金项目(81260225) 中国科学院重点实验室基金项目(CXJJ-14-S134);中国科学院“百人计划”择优项目资助
【分类号】：R312;TN24

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