利用原子力显微镜观察自体血回输对红细胞膜形貌的影响

发布时间：2018-06-17 02:01

本文选题：自体血回输 + 红细胞　；参考：《暨南大学》2012年硕士论文

【摘要】：目的利用原子力显微镜(AFM)观察自体血回输对红细胞形貌及膜超微结构的影响。方法选取择期行脊柱手术的患者10例，年龄35~65岁，ASAI~III级，所有患者均采用静脉全麻，术中按常规方法应用自体血液回收机。切皮前采集外周静脉血2ml（T1），采集蓄血罐中回收血即洗涤前血液2ml（T2），采集清洗完毕后，，输血袋内红细胞悬液即洗涤后血液2ml（T3），以上血液样本均用肝素抗凝。所有样本采用荧光正置显微镜放大400倍下观察红细胞，并计算其畸形率。利用AFM观察红细胞膜表面超微结构的变化以及红细胞膜表面粗糙度。结果（1）T1时刻红细胞畸形率（3.12±1.75）%低于T2（9.82±2.30）%和T3（9.62±2.67）%（P0.05）,而后两者之间并无统计学差异（P0.05）。（2）回收血和静脉血红细胞AFM形貌、红细胞膜表面蛋白颗粒分布均存在差异。T2红细胞膜表面平均粗糙度Ra（16.40±5.04）、均方根粗糙度Rq（20.24±5.89)高于T1（Ra：4.05±0.53；Rq：5.07±0.65）和T3（Ra：4.51±0.81；Rq：5.73±1.69）时刻收集的红细胞（P0.05），而后两者红细胞膜表面粗糙度并无统计学差异（P0.05）。结论自体血液回收可增加红细胞畸形率及改变红细胞AFM形貌及膜表面超微结构。
[Abstract]:Objective to observe the effect of autologous blood transfusion on erythrocyte morphology and membrane ultrastructure by atomic force microscope (AFM). Methods Ten patients (35 ~ 65 years old with ASAI III grade) undergoing spinal surgery were selected. All patients were treated with intravenous general anesthesia and autologous blood recovery machine was used in the operation. The peripheral venous blood 2ml / T _ 1 was collected before skin incision, and the blood was recovered from the blood storage tank before washing. After the collection and cleaning, the blood was washed with 2ml / T _ (3) of the red blood cell suspension in the blood transfusion bag, and the above blood samples were all treated with heparin anticoagulant. All samples were magnified 400 times by fluorescence positive microscope to observe red blood cells and calculate their deformity rate. The ultrastructure of erythrocyte membrane surface and the surface roughness of erythrocyte membrane were observed by AFM. Results at T1, the erythrocyte malformation rate was 3.12 卤1.75%, which was lower than that of T2P 9.82 卤2.30% and T3 9.62 卤2.67% P0.05, but there was no significant difference between the two groups. The mean surface roughness of erythrocyte membrane was 16.40 卤5.04, RqN 20.24 卤5.89), which was higher than that of T1 Raw 4.05 卤0.53 RQ: 5.07 卤0.65) and T3 Raw 4.51 卤0.81; RQ: 5.73 卤1.69) the erythrocyte membrane surface roughness of RQ: 5.73 卤1.69 was not significantly different from that of RQ: 5.73 卤1.69. Conclusion the recovery of autologous blood can increase the rate of erythrocyte malformation and change the morphology of erythrocyte AFM and the ultrastructure of membrane surface.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R329

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