脂多糖作用人蜕膜细胞后COX-2表达的影响

发布时间：2018-06-17 14:28

本文选题：LPS + COX-2　；参考：《中南大学》2011年硕士论文

【摘要】：目的：研究体外培养的人蜕膜细胞,经不同浓度脂多糖(LPS)作用后,蜕膜细胞中的炎性因子诱导酶环氧合酶2(COX-2)表达差异,探索COX-2过度表达与蜕膜细胞感染的相关性。方法：收集正常早孕期人工流产术中的蜕膜组织,进行体外原代细胞培养。当蜕膜细培养3-4代后将其分成5组,分别加入脂多糖使其终浓度分别为Ong/ml、25ng/ml、50ng/ml、100ng/ml、200ng/ml。作用蜕膜细胞48小时后,1)通过激光共聚焦-免疫荧光技术检测COX-2蛋白在蜕膜细胞中的表达差异2)在收集各组的蜕膜细胞提取mRNA,并采用半定量逆转录聚合酶链反应法(RT-PCR)检测各组中COX-2 mRNA的表达水平,并分析各组间的表达有无统计学差异。结果：1)通过激光共聚焦-免疫荧光染色技术检测到各组人蜕膜细胞后,细胞质及细胞核中均有COX-2蛋白表达,且加入LPS作用后各实验组与对照组相比,COX-2表达差异显著,P0.05,有统计学意义；且随着LPS浓度逐渐升高,COX-2蛋白表达逐渐增强。2)不同浓度LPS作用蜕膜细胞后,各实验组与对照组相比较,COX-2mRNA表达差异显著,P0.05,有统计学意义；且随着LPS浓度的增加,COX-2mRNA表达逐渐增强。结论：1)LPS作用于体外培养的人蜕膜细胞后,COX-2的蛋白表达增加,且随着LPS的浓度逐渐增加COX-2蛋白表达逐渐增强；2)LPS作用于体外培养的人蜕膜细胞后，，COX-2的mRNA表达增加,且随着LPS的浓度逐渐增加COX-2mRNA表达逐渐增强。
[Abstract]:Aim: to study the difference of expression of inflammatory factor inducible enzyme cyclooxygenase-2 (COX-2) in decidua cells treated with different concentrations of lipopolysaccharide (LPS) in vitro, and to explore the relationship between COX-2 overexpression and decidua cell infection. Methods: the decidual tissue of normal early pregnancy induced abortion was collected and cultured in vitro. When the decidua were cultured for 3 to 4 generations, they were divided into 5 groups, and the final concentration of lipopolysaccharide was 100 ng / ml ~ 100 ng / ml ~ (50) ng 路ml ~ (-1) ~ (-1), respectively. The difference of expression of COX-2 protein in decidual cells was detected by laser confocal immunofluorescence (2) mRNAs were extracted from decidua cells in each group and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) was used. The expression of COX-2 mRNA in each group was detected by RT-PCR. And analysis of the expression of statistical differences between the groups. Results the expression of COX-2 protein was detected in the cytoplasm and nucleus of decidua cells by laser confocal immunofluorescence staining. Compared with the control group, the expression of COX-2 in each experimental group was significantly different from that in the control group (P 0.05), and the expression of COX-2 protein increased gradually with the increase of LPS concentration. Compared with the control group, the expression of COX-2 mRNA in each experimental group was significantly higher than that in the control group (P 0.05), and increased with the increase of LPS concentration. Conclusion the expression of COX-2 protein in human decidua cells cultured in vitro was increased after treated with LPS, and the expression of COX-2 protein increased gradually with the increase of LPS concentration, and the expression of COX-2 mRNA in human decidual cells cultured in vitro was increased after lipopolysaccharide (LPS) treatment. The expression of COX-2 mRNA increased with the concentration of LPS.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R329

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