免疫球蛋白G在成年大鼠肝脏细胞中的表达

发布时间：2018-06-18 14:20

本文选题：肝细胞 + 免疫球蛋白G　；参考：《汕头大学》2011年硕士论文

【摘要】：背景和目的经典理论认为,免疫球蛋白G(immunoglobulin G)仅由分化成熟的B淋巴细胞合成。近几年来,越来越多的研究者表明,上皮来源的恶性肿瘤及其体外培养的细胞系中,如肝癌、乳腺癌等,也能产生并且分泌免疫球蛋白G。同时,很多研究者也发现,在一些正常的细胞中,也有免疫球蛋白G的表达。但是免疫球蛋白G是否能够在成熟的肝细胞中有所表达尚未阐明。本文主要研究在SD大鼠成熟的肝细胞中,免疫球蛋白G的表达情况。材料与方法 1.研究对象10例体重达250-300g雄性成年Sprague Dawley大鼠肝脏组织。该品系大鼠购于汕头大学医学院实验动物中心。 2.免疫组织化学染色(Immunohistochemistry,IHC)检测石蜡组织切片中免疫球蛋白G蛋白的表达。 3.原位杂交技术(in situ hybridization,ISH)检测石蜡组织切片中免疫球蛋白G mRNA的表达。 4.显微切割单一细胞RT-PCR技术(laser capture microdissection (LCM) related single-cell RT-PCR)检测新鲜组织中免疫球蛋白G mRNA的表达。结果 1.免疫组织化学在成年大鼠肝脏组织的石蜡切片中,肝脏细胞的胞浆中有免疫球蛋白G的染色信号。 2.原位杂交技术在成年大鼠肝脏组织的石蜡切片中,肝脏细胞的胞浆中有免疫球蛋白G的mRNA的表达。 3.显微切割单一细胞PCR技术显微切割提取的成年大鼠肝脏细胞中,有免疫球蛋白G的mRNA的表达。结论在成年大鼠的肝脏细胞中,有免疫球蛋白G蛋白和mRNA的表达。
[Abstract]:Background and objective the classical theory suggests that immunoglobulin G is only synthesized by mature B lymphocytes. In recent years, more and more researchers have shown that epithelial-derived malignant tumors and their cell lines in vitro, such as liver cancer, breast cancer, can also produce and secrete immunoglobulin G. At the same time, many researchers have also found that immunoglobulin G is expressed in some normal cells. However, it is not clear whether immunoglobulin G can be expressed in mature hepatocytes. The expression of immunoglobulin G in mature rat hepatocytes was studied in this paper. Materials and methods 1. Participants liver tissues of 10 adult Sprague Dawley rats weighing 250-300 g were studied. The strain was purchased from Experimental Animal Center, School of Medicine, Shantou University. Immunoglobulin G protein expression in paraffin sections was detected by immunohistochemical staining. 3. 3. The expression of immunoglobulin G mRNA in paraffin tissue sections was detected by in situ hybridization technique (ISH). 4. The expression of immunoglobulin G mRNA in fresh tissue was detected by microdissected single cell RT-PCR and related single-cell RT-PCR. Result 1. Immunohistochemical staining of immunoglobulin G was found in the cytoplasm of adult rat liver tissue in paraffin sections. 2. The expression of immunoglobulin G mRNA was detected in the cytoplasm of adult rat liver tissue by in situ hybridization. Immunoglobulin G mRNA was expressed in adult rat liver cells by microdissecting single cell PCR technique. Conclusion immunoglobulin G protein and mRNA are expressed in adult rat liver cells.
【学位授予单位】：汕头大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R392

【相似文献】