迷走神经切断及烟碱预处理对大鼠SIRS影响的初步研究

发布时间：2018-06-21 13:45

本文选题：两次打击 + 迷走神经切断　；参考：《遵义医学院》2011年硕士论文

【摘要】：目的：通过复制“二次打击”SIRS大鼠模型,探讨迷走神经切断及烟碱预处理对大鼠SIRS的作用,为临床治疗SIRS提供新思路。方法：选择雄性健康SD大鼠40只,随机分为四组,每组10只。对照组在诱导SIRS模型前7d未予处理；单侧迷走神经切断组：在诱导SIRS模型前7d,行右侧颈部迷走神经切断；烟碱预处理组：在诱导SIRS模型前7d,开始每天给予烟碱液5mg. kg-1ip;联合迷走神经切断+烟碱预处理组：在诱导SIRS模型前7d,联合迷走神经切断+烟碱预处理。单侧迷走神经切断术采用腹颈正中切口显露,切断右侧颈部迷走神经主干。造模时采用腹腔注射麻醉,制作“二次打击”(休克及腹腔内注入LPS)动物模型。采集血液标本或器官组织进行相关检测。检测结果进行统计学分析处理,比较四组大鼠TNF-a、IL-6含量；外周血白细胞计数与分类；血生化及肺、肝、肾的病理学变化。结果：迷走神经切断后能提高炎症反应并加重大鼠重要脏器的损伤。烟碱预处理后能降低炎症反应,并对重要脏器有保护作用。联合处理后炎症反应及重要脏器损伤程度介于上述两者之间,与对照组比较仍有统计学意义。结论：中枢神经能够通过副交感神经途径调节炎症反应；烟碱能抗炎且对重要脏器有一定保护作用,其可不依赖于迷走神经的完整性而独立发挥作用。
[Abstract]:Objective: to study the effect of vagotomy and nicotine pretreatment on Sirs in rats by making "second strike" Sirs rat model, and to provide a new idea for clinical treatment of Sirs. Methods: forty male healthy SD rats were randomly divided into four groups with 10 rats in each group. In the control group, the Sirs model was not treated 7 days before induction, and in the unilateral vagotomy group, the right cervical vagotomy was performed 7 days before the Sirs model was induced. Nicotine pretreatment group: 7 days before Sirs model was induced, 5 mg 路kg ~ (-1) IP of nicotine solution was given daily, and 7 days before induction of Sirs model, combined vagotomy nicotine pretreatment group was combined with vagus nerve transection nicotine pretreatment group. Unilateral vagotomy was performed by median incision of abdominal neck and transection of right cervical vagus nerve trunk. The animal model of second strike (shock and intraperitoneal injection of LPSs) was made by intraperitoneal injection of anesthesia. Blood samples or organ tissues were collected for relevant examination. The results were statistically analyzed to compare the contents of TNF-a IL-6, the count and classification of peripheral blood leukocytes, the pathological changes of blood biochemistry, lung, liver and kidney in the four groups. Results: vagotomy can increase the inflammatory response and aggravate the injury of important organs in rats. Nicotine pretreatment can reduce inflammation and protect important organs. The degree of inflammatory reaction and injury of important organs after combined treatment was between the above two groups, which was still statistically significant compared with the control group. Conclusion: CNS can regulate inflammatory response through parasympathetic pathway, nicotine can resist inflammation and protect important organs, and it can play an independent role independent of the integrity of vagus nerve.
【学位授予单位】：遵义医学院
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363

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