结核特异性多肽E6、E7和C14对单核-巨噬细胞亚型极化的影响

发布时间：2018-06-22 10:14

本文选题：肽类 + 白血病　；参考：《中国医药生物技术》2016年03期

【摘要】：目的运用流式细胞术检测单核-巨噬细胞极化亚型M1(CD14~+CD16~+)及M2(CD14~+CD163~+),探讨结核特异性多肽对单核-巨噬细胞极化分型的影响。方法以3种结核特异性多肽E6、E7和C14作为刺激物,刺激人急性白血病单核细胞株(THP-1细胞)、结核病患者胸水单个核细胞及外周血单个核细胞(PBMC),用流式细胞术检测刺激物不同时间点对单核-巨噬细胞极化表面标记表达的影响。结果结核特异性多肽E6刺激THP-1 24+0 h、24+24 h、24+48 h、24+72 h后,CD16/CD163阳性率分别为16.85%/13.78%、19.59%/15.68%、18.14%/14.19%、13.61%/11.47%。E7刺激效果与E6相似,C14对THP-1的CD16/CD163表达影响不如前两种多肽强。结核特异性多肽E6刺激结核患者胸水或血标本单核细胞19 h后,CD14+CD16+阳性率(1#患者:1.66%,2#患者:4.37%)与CD14~+CD163~+阳性率(1#患者:1.76%,2#患者:2.82%)差异不大,但CD14~+CD16~+CD86~+阳性率(1#患者:2.20%,2#患者:6.16%)大于CD14~+CD163~+CD206~+阳性率(1#患者:1.37%,2#患者:0.92%)。E7刺激效果与E6相似,C14对结核患者胸水或血标本单核细胞CD14/CD16/CD163表达影响不如前两种多肽强。结论 3种结核特异性多肽特别是E6、E7体外刺激单核细胞株THP-1、结核患者胸水或血标本单核细胞主要向M1型单核-巨噬细胞极化。
[Abstract]:Objective to investigate the effect of tuberculosis specific polypeptides on the polarization typing of mononuclear macrophages by using flow cytometry to detect the polarization subtypes M1 (CD14 ~ CD16 ~) and M2 (CD14 ~ CD163 ~) of monocyte-macrophage. Methods three TB specific peptides, E6, E7 and C14, were used as stimulators. Human acute leukemia monocytes (THP-1 cells), pleural effusion mononuclear cells (PBMC) and peripheral blood mononuclear cells (PBMC) were stimulated. Results the positive rate of CD16 / CD163 was 16.85 / 13.78 / 19.59 / 18.14 / 14.19.1 / 13.61 / 11.47.E7 similar to that of E6. The positive rate of CD16 / CD163 in THP-1 was similar to that of E6. The expression of CD16 / CD163 in THP-1 was not significantly affected by C14 as compared with that of E6. The positive rates of CD14 CD16 in pleural effusion or blood samples stimulated by TB specific polypeptide E6 for 19 h were not significantly different from those of CD 14 ~ CD 163 ~ + in patients with tuberculosis (n = 1.662#: 4.37%) and those in patients with CD _ (14) ~ CD _ (163) ~ (2. 82%). However, the positive rate of CD14 ~ CD16 ~ CD86 ~ + was higher than that of CD14 ~ CD163 ~ CD206 ~ (2. 16%). The effect of E7 stimulation was similar to that of E6 on the expression of CD14 / CD16 / CD163 in pleural effusion or blood samples of TB patients. Conclusion the monocytes of THP-1 were stimulated by three tuberculous specific polypeptides, especially E6G E7 in vitro. The monocytes in pleural effusion or blood samples of TB patients were mainly polarized to M1 type mononuclear macrophages.
【作者单位】：中山大学中山医学院微生物学教研室热带病防治研究教育部重点实验室结核病防治研究所海洋微生物功能分子广东省高校重点实验室广东省重大传染病预防和控制技术中心;广州市胸科医院;
【基金】：国家自然科学基金面上项目(81271779) “十二五”防治传染病科技重大专项分任务(2012ZX10004903-004-002) 广东省自然科学基金(2014A030313725) 广州市科创委产学研协同创新重大专项(2060404)
【分类号】：R392

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