人脐带间充质干细胞对类噬血细胞性淋巴组织细胞增多症小鼠模型肝脏损伤的修复作用及机制

发布时间：2018-06-22 12:14

本文选题：人脐带间充质干细胞 + 噬血细胞性淋巴组织细胞增多症　；参考：《第三军医大学学报》2016年17期

【摘要】：目的探讨人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,h UC-MSCs)对类噬血细胞性淋巴组织细胞增多症(hemophagocytic lymphohistocytosis,HLH)小鼠模型肝脏损伤的修复作用及相关机制。方法采用h UC-MSCs的体外标记,将成年野生型C57BL/6小鼠54只,按完全随机方法分为模型损伤组(PBS组)、干细胞治疗组(MSC组)、正常对照组(CON组)。类HLH小鼠模型采用CPG-ODN1826联合IFN-γ诱导产生,MSC组治疗采用h UC-MSCs经尾静脉移植。观察移植后第1、3、7天小鼠一般情况,血生化(ALT、AST、ALB、TG),外周血三系(WBC、RBC、PLT、HGB)变化,第7天肝质量体质量比,HE染色观察肝脏组织病理变化,免疫组化法观察第7天肝脏中活化巨噬细胞的数量,荧光显微镜观察MSC组CM-Dil标记的h UC-MSCs的肝内定植,免疫荧光法观察第7天MSC组肝脏中抗人白蛋白抗体阳性细胞的数量。结果与PBS组相比,MSC组小鼠的状态好转,肝质量体质量比显著下降(P0.05);ALT、AST、TG显著降低(P0.05),ALB无明显升高(P0.05),WBC、PLT、RBC、HGB显著升高(P0.05);肝脏炎症浸润减少、肝组织损伤恢复;肝脏中活化巨噬细胞数量明显减少(P0.05);肝脏中均可发现有CM-Dil标记的h UC-MSCs的定植,且无人白蛋白的表达。结论 CM-Dil标记的h UC-MSCs经尾静脉移植可以在类HLH小鼠受损的肝脏中定植,并可改善肝功能、修复肝结构、降低活化巨噬细胞的数量,且不是通过h UC-MSCs肝样转化实现的。
[Abstract]:Objective to investigate the repair effect of human umbilical cord mesenchymal stem cells (human umbilical cord mesenchymal stem cells) on liver injury in hemophagocytic lymphoid histiocytosis (HLH) mice. Methods 54 adult wild-type C57BL / 6 mice were labeled with hUC-MSCs in vitro and randomly divided into model injury group (PBS group), stem cell therapy group (MSC group) and normal control group (Con group). HLH-like mouse model was treated by tail vein transplantation of hUC-MSCs induced by CPG-ODN1826 and IFN- 纬. The changes of blood biochemistry (alt) and peripheral blood triplex (WBC) were observed on the 7th day after transplantation. The pathological changes of liver tissue were observed by HE staining on the 7th day, and the number of activated macrophages in the liver on the 7th day was observed by immunohistochemical method. The intrahepatic colonization of hUC-MSCs labeled with CM-Dil in MSC group was observed by fluorescence microscope, and the number of anti-albumin antibody positive cells in liver of MSC group was observed by immunofluorescence method on the 7th day. Results compared with PBS group, the condition of MSC group was improved, the liver mass ratio was significantly decreased (P0.05), the ALB level was not significantly increased (P0.05), the liver inflammation infiltration was decreased, liver tissue injury was recovered, and there was no significant increase in ALB (P0.05). The number of activated macrophages in the liver was significantly decreased (P0.05), and CM-Dil labeled hUC-MSCs were found in the liver with no albumin expression. Conclusion CM-Dil labeled hUC-MSCs can be transplanted into the damaged liver of HLH-like mice through tail vein, and can improve liver function, repair liver structure and reduce the number of activated macrophages.
【作者单位】：重庆医科大学附属儿童医院血液科儿童发育疾病研究教育部重点实验室儿童发育重大疾病国家国际科技合作基地重庆市干细胞治疗工程技术研究中心;
【分类号】：R-332;R725.5

【相似文献】