高脂饮食结合STZ诱导妊娠期糖尿病小鼠模型的建立

发布时间：2018-06-23 17:33

本文选题：妊娠期糖尿病 + 高脂饮食　；参考：《中国比较医学杂志》2016年03期

【摘要】：目的探讨稳定、可靠与妊娠期胰岛素抵抗相似的小鼠模型建立方法。方法将60只SPF级5周龄KM小鼠随机分为高脂饮食组和普食组,高脂饮食组高脂饲料喂养4周后,按雌雄1∶1合笼,在雌鼠阴道看到阴道栓定位妊娠第一天。妊娠成功后,按30 mg/kg间隔24 h腹腔注射,共注射3次,对照组注射等量的柠檬酸缓冲液(0.1 mol/L,p H=4.2)。于造模成功后的第3、7、14及19天记录小鼠的随机血糖,体重,以及24 h的饮水量和摄食量。ELISA检测血清中INS、ADP、LEP和CRP因子的浓度。结果孕鼠在造模成功后出现明显饮水量、摄食量、尿量增多症状,饮水量、摄食量与对照组相比有显著差异(P0.01),造模组血糖11.1 mmol/L,明显高于对照组。GDM组INS(1.50±0.25)Mu/L,ADP(0.65±0.13)μg/L,LEP(1.60±0.12)μg/L,CRP(37.54±4.70)μg/L,与对照组相比差异极显著(P0.01)。产后,小鼠血糖恢复到正常水平。结论高脂饮食结合小剂量STZ多次诱导的方法能够建立的GDM模型且符合人类妊娠期糖尿病病理性胰岛素抵抗的特征。
[Abstract]:Objective to establish a stable and reliable mouse model of insulin resistance in pregnancy. Methods 60 SPF 5-week-old km mice were randomly divided into high fat diet group and general diet group. After feeding with high fat diet for 4 weeks, the mice were caged at 1:1 according to female and male, and the vaginal suppository was located on the first day of pregnancy in female mice. After pregnancy, the rats were injected intraperitoneally at intervals of 30 mg/kg and 24 h, respectively. The control group was injected with the same amount of citric acid buffer (0.1 mol / L H4. 2). The random blood glucose, body weight, 24 h drinking water and food intake. Elisa were used to detect the concentration of INSDA PLEP and CRP in the serum of the mice on the 3rd day, 714 and 19 days after the establishment of the model. Results there were significant differences in the quantity of drinking water, the amount of food intake, the quantity of urine, the quantity of drinking water and the amount of food consumed between the pregnant rats and the control group (P0.01). The blood glucose of the model group (11.1 mmol / L) was significantly higher than that of the control group (1.50 卤0.25) MuLP / ADP (0.65 卤0.13) 渭 g / L LLEP (1.60 卤0.12) 渭 g / L CRP (37.54 卤4.70) 渭 g / L, and that of the model group was significantly higher than that of the control group (P 0.01). Postpartum, blood sugar in mice returned to normal level. Conclusion High fat diet combined with low dose STZ induced GDM model can be used to establish GDM model and accord with the characteristics of insulin resistance in gestational diabetes mellitus.
【作者单位】：扬州大学医学院;泰兴市人民医院;
【基金】：国家自然科学基金资助项目(81272537;30801497) 江苏省高校自然科学基金资助项目(11KJB360010) 扬州大学“新世纪人才工程”资助(2012)
【分类号】：R714.256;R-332

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