大鼠肠缺血再灌注损伤后TLR2的改变与肠黏膜屏障损伤的关系

发布时间：2018-06-23 21:47

本文选题：肠缺血再灌注 + 肠黏膜屏障　；参考：《延边大学》2011年硕士论文

【摘要】：[目的]复制大鼠肠缺血再灌注损伤模型,探讨TLR2在大鼠肠缺血再灌注损伤中的改变及其意义。旨在求证肠缺血再灌注损伤后小肠的广泛炎性损伤及后续的SIRS或MODS与小肠TLR2介导的天然免疫间关系。[方法]采用肠系膜上动脉夹闭法建立小肠缺血再灌注模型,将60只健康Wistar大鼠随机分为正常对照组A(6只)、假手术组B(6只)和小肠缺血再灌注(IIR)损伤模型组(48只),其中根据肠系膜上动脉的不同夹闭时间(30min、40min、50min)分为C-E组,并计算大鼠的24小时内死亡率并找出模型的最佳夹闭时间段。之后用此夹闭时间造出模型,并分为(2h、6h、12h、24h、48h)F-J等5组在各组的相应时间取材,用酶联免疫吸附法(ELISA)检测各组织中的DAO和TLR2含量,并且取部分小肠做HE染色做比较。[结果]IIR后可见HE染色的肠组织中有明显改变,并.且大鼠血清、小肠组织、peyer淋巴结及肝组织的TLR2和血清中DAO的含量与正常对照组相比均明显增加(p0.05)。TLR2与DAO的改变有相关性；peyer淋巴结中有TLR2的表达,并且与正常对照组相比有升高(p0.05),但与其他组织中TLR2反应较慢。[结论]肠缺血再灌注损伤可导致多种组织TLR2表达增强,其改变与肠源性感染发生、发展和肠粘膜屏障的破坏有关,其机制可能与小肠黏膜TLR2炎症介质信号传导通路全面激活,所引起的天然免疫反应及获得性免疫反应有关。
[Abstract]:[objective] to study the changes and significance of TLR2 in intestinal ischemia reperfusion injury in rats. The aim of this study was to identify the extensive inflammatory injury of small intestine following intestinal ischemia-reperfusion injury and the relationship between Sirs or mods and TLR2 mediated innate immunity in the small intestine. [methods] the intestinal ischemia-reperfusion model was established by clamping the superior mesenteric artery. Sixty healthy Wistar rats were randomly divided into normal control group (n = 6), sham-operated group B (n = 6) and intestinal ischemia-reperfusion (IIR) model group (n = 48). According to the different clamping time of superior mesenteric artery (30 min, 40 min or 50 min), the rats were divided into C-E group. The 24-hour mortality of rats was calculated and the optimal clamping time of the model was found. Then the model was made with this clamping time, and was divided into 5 groups (2 h ~ 6 h ~ 12 h ~ 24 h ~ 48 h) F-J and so on. The contents of Dao and TLR2 in tissues were detected by enzyme linked immunosorbent assay (Elisa), and some small intestine were stained with HE for comparison. [results] after IIR, there were obvious changes in the intestinal tissue stained by HE. The levels of TLR2 and Dao in serum, small intestinal lymph nodes and liver tissues in rats were significantly higher than those in normal controls (p0.05) .TLR2 and Dao were correlated with the expression of TLR2 in the lymph nodes of rats. Compared with the normal control group, TLR2 was increased (p0.05), but the TLR2 reaction was slower than that in other tissues. [conclusion] intestinal ischemia-reperfusion injury can result in increased expression of TLR2 in various tissues. The changes of TLR2 expression may be related to the occurrence and development of enterogenic infection and the destruction of intestinal mucosal barrier. The mechanism may be related to the activation of TLR2 inflammatory signal transduction pathway in intestinal mucosa. The innate immune response and the acquired immune response are related.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363

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