东莨菪碱与苯扎氯铵干眼症大鼠造模方法研究

发布时间：2018-06-24 19:47

  本文选题：干眼症 + 造模方法　； 参考：《动物医学进展》2017年10期

【摘要】：为比较氢溴酸东莨菪碱皮下注射与苯扎氯铵滴眼制作干眼症动物模型的各自特点,将健康SD大鼠随机分为正常组、东莨菪碱加风组、苯扎氯铵组和吹风对照组。正常组不接受任何处理;东莨菪碱组皮下注射氢溴酸东莨菪碱注射剂;苯扎氯铵组以2mg/mL苯扎氯铵滴眼液每日3次滴眼;吹风组仅置于吹风装置中每日12h。于试验第3、7、10天检查Schirmer I试验(SIT)、泪膜破裂时间(BUT)、角膜荧光素染色(FLS)。结果表明,东莨菪碱组在第3、7、10天,SIT与正常组相比具有显著差异(P0.05),第7、10天BUT和FLS与正常组相比具有显著差异(P0.05);苯扎氯铵组在试验第7、10天,SIT试验和BUT与正常组相比有显著差异(P0.05),BUT第10天差异达到最大,在第3、7、10天,FLS与正常组相比具有显著差异(P0.05);组织病理学检查东莨菪碱组泪腺较苯扎氯铵组明显有腺上皮细胞萎缩;苯扎氯铵组大鼠角结膜上皮细胞明显变薄,表层细胞排列紊乱,细胞数量显著减少,并存在炎性细胞浸润。提示造模10d氢溴酸东莨菪碱注射剂皮下注射配合吹风制作的干眼症动物模型对大鼠泪腺及眼表上皮细胞影响显著,伴有较轻程度的眼表损伤,更适合早期轻中度干眼造模;苯扎氯铵滴眼造模方法易造成眼表上皮损伤,适合短期重度干眼的造模。
[Abstract]:In order to compare the characteristics of scopolamine hydrobromide subcutaneous injection and benzalkonium chloride dropping eye to make dry eye animal model, healthy SD rats were randomly divided into normal group, scopolamine plus wind group, benzalkonium chloride group and blow control group. Scopolamine hydrobromide injection was injected subcutaneously in scopolamine group, 2 mg / mL benzalkonium chloride eye drops were used three times a day in the benzalkonium chloride group, and only 12 hours a day in the blowing group. Schirmer I test (sit), tear film rupture time (but) and corneal fluorescein staining (FLS) were examined on day 3. The results show that There was significant difference between scopolamine group and normal group on day 3 (P0.05), but and FLS on day 7 (P0.05), and that of sit and but on day 710 (P0.05) in benzalkonium chloride group (P0.05), and that of but on day 710 in anisolamine group was significantly higher than that in normal group (P0.05), and that of but in group B was significantly higher than that in group B on day 710 (P0.05), and that of but in group B was significantly higher than that in group B on day 710 (P0.05). The difference reached the maximum on the 10th day, There was significant difference between FLS and normal group on the 3rd 7th day (P0.05). Histopathological examination showed that the lacrimal gland of scopolamine group had obvious atrophy of glandular epithelium than that of benzalkonium chloride group, the epithelial cells of cornea conjunctiva of rats in benzalkonium chloride group were thinning obviously, and the surface cells were disordered. The number of cells decreased significantly and inflammatory cell infiltration occurred. It is suggested that the animal model of dry eye induced by injection of scopolamine hydrobromide injection and blowing air for 10 days has a significant effect on lacrimal gland and ocular surface epithelial cells, which is more suitable for early dry eye model. The method of eye modeling with benzalkonium chloride dripping is easy to cause ocular surface epithelium injury and is suitable for short term dry eye modeling.
【作者单位】： 上海中医药大学;上海市针灸经络研究所;北京中医药大学;
【基金】：上海市卫生和计划生育委员会项目(№201440412,ZJ2016028) 上海市自然科学基金项目(№13ZR1439500)
【分类号】：R-332;R777.34
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本文编号：2062711