下丘脑—海马Ghrelin神经元投射及海马微量注射Ghrelin对大鼠摄食、体重及胃运动的影响

发布时间：2018-06-25 14:59

本文选题：海马 + 逆行追踪　；参考：《青岛大学》2011年硕士论文

【摘要】：目的：观察下丘脑室旁核(PVN)-海马Ghrelin神经元的投射;探讨海马微量注射Ghrelin对大鼠摄食、体重、胃运动的影响。方法：采用荧光金(Fluorogold, FG)逆行追踪结合荧光免疫组化方法,探讨下丘脑PVN Ghrelin神经元向海马的投射,以及海马神经元向下丘脑PVN的投射；采用海马核团微量注射Ghrelin,观察清醒大鼠摄食、体重及胃运动的改变。结果：海马微量注射FG,7天后可见下丘脑PVN有大量标记FG的神经元,且呈同侧优势表达,结合Ghrelin荧光免疫组化实验,发现部分下丘脑PVN FG神经元有Ghrelin免疫阳性物的表达；下丘脑PVN微量注射FG,7天后可见海马CA1、CA2-3区有大量FG标记的神经元,且呈同侧优势表达。海马CA1区微量注射Ghrelin后,大鼠摄食和体重明显增加(P0.05),胃运动明显增强(P0.05),且呈显著剂量依赖关系。结论：下丘脑PVN-海马有往返纤维投射,且呈同侧优势投射；下丘脑PVN Ghrelin免疫阳性神经元投向海马CA1区；海马CA1区Ghrelin免疫阳性神经元投向下丘脑PVN；海马CA1区Ghrelin通过其受体参与大鼠摄食、体重和胃运动的调控。
[Abstract]:Aim: to observe the projections of hypothalamic paraventricular nucleus (PVN)-hippocampal ghrelin neurons and to investigate the effects of hippocampal microinjection of ghrelin on feeding weight and gastric motility in rats. Methods: the projections of hypothalamic PVN ghrelin neurons to hippocampus and hippocampal neurons to hypothalamic PVN were studied by using Fluorogold (FG) retrograde tracing and fluorescence immunohistochemistry. Hippocampal nuclei were microinjected with ghrelin to observe the changes of food intake, body weight and gastric motility in conscious rats. Results: a large number of neurons labeled with FG were found in hypothalamus PVN 7 days after microinjection of FGG, and the expression of FG was ipsilateral dominant. Combined with ghrelin fluorescence immunohistochemical experiment, ghrelin immunoreactivity was found in some hypothalamic PVN FG neurons. 7 days after microinjection of hypothalamic PVN, a large number of FG labeled neurons were found in hippocampal CA1P CA2-3 area, and showed ipsilateral dominant expression. After microinjection of ghrelin in hippocampal CA1 area, the feeding and body weight of the rats increased significantly (P0.05), and the gastric motility increased significantly (P0.05) in a dose-dependent manner. Conclusion: hypothalamic PVN- hippocampal neurons have homolateral projections, hypothalamic PVN ghrelin immunoreactive neurons turn to hippocampal CA1 region, hippocampal CA1 region ghrelin immunoreactive neurons into hypothalamic PVNs. Ghrelin in hippocampal CA1 region participates in the regulation of feeding, body weight and gastric motility through its receptor.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363

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