DBD等离子体在生物医学中的应用研究

发布时间：2018-06-27 11:06

本文选题：DBD等离子体 + 聚四氟乙烯　；参考：《长春理工大学》2011年硕士论文

【摘要】：介质阻挡放电(DBD)产生的低温等离子体在生物医学中的应用是一个跨学科的热点课题,本论文根据介质阻挡放电的原理,使用新型大气压DBD等离子体发生装置,对生物医学中两个方面的应用进行了可行性研究。聚四氟乙烯(PTFE)具有低表面自由能和化学惰性的特点,使其成为生物医学中应用最广泛的一种材料。使用大气压DBD等离子体的方法改善聚四氟乙烯表面亲水性,通过测量亲水角、扫描电子显微镜(SEM)观察和X射线光电子能谱(XPS)分析,研究了处理前后PTFE表面性能的变化,结果表明,PTFE表面氧原子百分率由0增加到12.89,表面亲水角从115°下降至42°。利用大气压DBD等离子体处理体外肺癌细胞(A549),对其灭活机理进行初步研究。研究表明等离子体处理肺癌细胞的过程中,培养基温度不会超过314K,对细胞存活率不会产生影响,而等离子体产生的高速粒子、自由基以及紫外线均对细胞活性产生影响。采用中性红摄入法对肺癌细胞进行染色,观察处理前后细胞形态、密度的不同,测量处理前后OD值的变化。选用不同气体放电,总结出氩气中掺入2.5%的氧气放电时,能够激发更多的自由基的自由电子,杀灭肺癌细胞的效果最为理想。
[Abstract]:The application of low temperature plasma generated by dielectric barrier discharge ( dbd ) in biomedical is a cross - disciplinary hot topic . According to the principle of dielectric barrier discharge , this paper makes a feasibility study on two aspects of biological medicine by using a novel atmospheric plasma generating device .

Polytetrafluoroethylene ( PTFE ) has the characteristics of low surface free energy and chemical inertness , which makes it become one of the most widely used materials in biomedical applications . By measuring hydrophilic angle , scanning electron microscope ( SEM ) observation and X - ray photoelectron spectroscopy ( XPS ) , the change of surface properties of PTFE is studied by measuring hydrophilic angle , scanning electron microscope ( SEM ) and X - ray photoelectron spectroscopy ( XPS ) . The results show that the percentage of oxygen atoms in PTFE surface is increased from 0 to 12.89 , and the hydrophilic angle of surface decreases from 115 掳 to 42 掳 .

In vitro lung cancer cells ( A549 ) were treated by atmospheric pressure plasma treatment . The mechanism of inactivation of lung cancer cells was studied . The results showed that the medium temperature did not exceed 314K during the treatment of lung cancer cells .
【学位授予单位】：长春理工大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R312;O539

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