诱导多能干细胞的安全性及应用研究进展

发布时间：2018-06-28 17:37

本文选题：干细胞 + 多能干细胞　；参考：《中国组织工程研究》2017年05期

【摘要】：背景:诱导多能干细胞技术解决了伦理学和免疫排斥等矛盾,高效安全定向诱导多能干细胞技术成了当今研究的主流。目的:综述诱导多能干细胞的诱导安全性和应用进展。方法:应用计算机检索Pub Med及相关期刊论文2006年1月至2016年3月有关诱导多能干细胞的文章,英文检索词为"induced pluripotent stem cell,reprogramming,clinical application,safety,transcription factor,disease model",中文检索词为"诱导多能干细胞,重编程,临床应用,遗传安全性,转录因子,疾病模型"。结果与结论:近年来诱导多能干细胞的研究备受科研界和医学界的关注,并努力克服了免疫和伦理问题,成功获得了诱导多能干细胞。但由于无法解决诱导多能干细胞技术中的安全性、效率和诱导多能干细胞细胞再分化机制等方面的问题,而局限于理论和实验室研究,迟迟无法应用于临床。如何安全高效的将诱导多能干细胞诱导为患者需要的类型并进行移植,如何建立良好的疾病模型与高通量药物筛选平台,涉及各方面的基础研究,面临着巨大的困难和挑战。
[Abstract]:Background: induced pluripotent stem cell technology has solved the contradiction between ethics and immune rejection. High efficient and safe directional induction of pluripotent stem cell technology has become the mainstream of current research. Objective: to review the safety and application of induced pluripotent stem cells. Methods: a computer-based search was conducted for articles on induced pluripotent stem cells from January 2006 to March 2016 in Pub Med and the full text database of Chinese periodicals. The key words in English were "induced pluripotent stem cell programming" and "induced pluripotent stem cells, reprogrammed" in Chinese, the key words were "induced pluripotent stem cells, reprogrammed", and the Chinese key words were: "induced pluripotent stem cells", "induced pluripotent stem cells" and "induced pluripotent stem cells". Clinical applications, genetic Safety, Transcriptional factors, Disease models. Results & conclusion: in recent years, the research of induced pluripotent stem cells has attracted the attention of the scientific and medical circles, and has successfully obtained the induced pluripotent stem cells by overcoming the immune and ethical problems. However, the problems of safety, efficiency and redifferentiation mechanism of induced pluripotent stem cells can not be solved, but limited to theoretical and laboratory studies, so they can not be applied in clinical practice. How to safely and efficiently induce induced pluripotent stem cells into patients' need type and transplant, how to establish a good disease model and high-throughput drug screening platform, involving various aspects of basic research, facing great difficulties and challenges.
【作者单位】：海南医学院第一附属医院;
【基金】：国家国际科技合作专项(2014DFA30180) 国家科技部973项目(2012CB966502) 国家自然科学基金(81060175,30860103,81460034,81260032,81060016,31140021) 海南省国际科技合作专项(GJXM20100004,GJXM201106,KJHZ2014-11) 人力资源和社会保障部2011年度留学人员科技活动择优资助项目教育部归国留学人员启动基金项目海南医学院创新性实验项目(HYCX201304)~~
【分类号】：R329.2

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