两种冠状动脉结扎法制作大鼠急性心肌梗死模型的比较

发布时间：2018-07-01 11:14

本文选题：心肌梗死 + 冠状动脉结扎　；参考：《国际心血管病杂志》2016年02期

【摘要】：目的:比较两种制作大鼠急性心肌梗死模型的冠状动脉结扎法,推荐一种稳定性高、生存率高的模型制作方法。方法:将40只SD大鼠按照随机数字法分为两组:常规组和改进组,比较两组的手术时间、生存率,术后24h测量两组大鼠心肌梗死面积,危险区面积以危险区占左室面积的百分比(Ar/LV)表示,心肌梗死面积用梗死区占危险区面积的百分比(An/Ar)表示,比较两组Ar/LV和An/Ar。结果:术后3h改进组和常规组的存活率分别为90%和60%,二者比较无统计学差异(P0.05);术后24h改进组大鼠的存活率为85%,常规组为45%,两组间有显著的统计学差异(P0.05);常规组Ar/LV和An/Ar分别为51.4%±2.5%和52.5%±2.4%,改进组Ar/LV和An/Ar分别为51.5%±2.3%和52.5%±2.2%,两组间均无统计学差异(P0.05);常规组的操作时间明显长于改进组,为(627.9±16.0)s对(253.9±12.7)s(P0.01)。结论:改进的模型制作方法提高了大鼠生存率,缩短了手术时间,具有显著优越性,值得推广。
[Abstract]:Aim: to compare the two methods of coronary artery ligation (CAL) in acute myocardial infarction (AMI) rats. Methods: forty Sprague-Dawley rats were randomly divided into two groups: routine group and improved group. The operation time, survival rate and myocardial infarction area were measured 24 hours after operation. The area of risk area was expressed as the percentage of risk area to left ventricular area (Ar-LV), and the area of myocardial infarction was expressed as the percentage of infarcted area to risk area (An/Ar). Results: the survival rates of the improved group and the routine group were 90% and 60%, respectively (P0.05), the survival rate of the improved group was 85% at 24 hours after operation, and that of the routine group was 45% (P0.05), and there was a significant difference between the two groups (P0.05). And An/Ar were 51.4% 卤2.5% and 52.5% 卤2.4% respectively. The Ar-LV and An/Ar of the improved group were 51.5% 卤2.3% and 52.5% 卤2.2%, respectively. There was no significant difference between the two groups (P0.05), the operation time of the routine group was significantly longer than that of the improved group. It was (627.9 卤16.0) s pair (253.9 卤12.7) s (P0.01). Conclusion: the improved model making method can improve the survival rate and shorten the operation time of rats. It has obvious advantages and is worth popularizing.
【作者单位】：河南中医学院第一附属医院心脏中心;河南中医学院第一附属医院中心实验室;
【基金】：国家自然科学青年基金支持项目(81303073) 河南省科技攻关重点项目(112102310317) 河南省高校科技创新团队支持计划(13IRTSTHN012) 河南省中医药防治心血管疾病创新型科技团队(C20130050) 河南中医学院省属科研业务专项(2014KYYWF-YQ12);河南中医学院一附院博士项目(2012KJ32)
【分类号】：R542.22;R-332

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