提高扩张型心肌病动物模型的生存率（英文）

发布时间：2018-07-01 12:49

本文选题：阿霉素 + 扩张型心肌病　；参考：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2016年12期

【摘要】：目的:比较在腹腔注射相同剂量阿霉素(12 mg/kg)下,不同给药方案构建扩张型心肌病大鼠模型的生存率。创新点:首次应用腹腔注射阿霉素1 mg/kg,一周两次的方法构建扩张型心肌病大鼠模型,并与常规的方法进行生存率的比较。方法:将150只SD雄鼠分为对照组(腹腔注射生理盐水)、Dox 1组(腹腔注射阿霉素1 mg/kg一周两次,共六周)和Dox 2组(腹腔注射阿霉素2 mg/kg一周一次,共六周)。观察及比较各组大鼠的体重、生存率、心腔大小、pro-BNP、CRP、CREA、AST和Caspase-3 mR NA水平。观察各组大鼠心肌病理变化,同时进行~(18)FDG-PET心肌代谢显像。结论:本实验中结果显示,Dox 1组和Dox 2组在pro-BNP、Caspase-3 mR NA水平、心肌病理变化及~(18)FDG-PET心肌代谢显像上均没有明显差异;但Dox 1组的生存率是78%,而Dox 2组的生存率是52%;同时,Dox 2组大鼠的血清CREA、AST和CRP水平比Dox 1组明显升高。综上所述,腹腔注射阿霉素1 mg/kg一周两次的方法在成功构建扩张型心肌病大鼠模型的同时大大提高了存活率。
[Abstract]:Aim: to compare the survival rate of dilated cardiomyopathy (DCM) rats with different administration regimen under the same dose of adriamycin (12 mg/kg). Innovation: the rat model of dilated cardiomyopathy was established by intraperitoneal injection of adriamycin (1 mg / kg) twice a week for the first time, and the survival rate was compared with the conventional method. Methods: 150 male SD rats were divided into control group (intraperitoneal injection of normal saline) and dox1 group (adriamycin 1 mg/kg once a week for six weeks) and dox2 group (adriamycin 2 mg/kg once a week for six weeks). The body weight, survival rate and the levels of caspase-3 mRNA and caspase-3 mRNA were measured and compared. Myocardial pathological changes were observed and ~ (18) FDG-PET myocardial metabolism imaging was performed. Conclusion: the results of this experiment showed that there was no significant difference between Dox1 group and Dox2 group in pro-BNPN Caspase-3 mRNA level, myocardial pathological changes and ~ (18) FDG-PET myocardial metabolism imaging. However, the survival rate of Dox1 group was 78%, while that of Dox2 group was 522.The serum creatine AST and CRP levels in Dox2 group were significantly higher than those in Dox1 group. In conclusion, intraperitoneal injection of adriamycin 1 mg/kg twice a week improved the survival rate of dilated cardiomyopathy (DCM) rats.
【作者单位】： Wuxi
【基金】：supported by the Medical Technology Major Projects on Hospital Management Center in Wuxi City,Jiangsu Province(No.YGZX1102),China
【分类号】：R542.2;R-332

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