吸烟大鼠实验性牙周炎动物模型的建立

发布时间：2018-07-04 15:20

本文选题：吸烟 + 大鼠　；参考：《青岛大学》2011年硕士论文

【摘要】：目的：通过观察结扎丝结扎吸烟大鼠上颌第二磨牙牙颈部后牙周组织的变化,探讨建立吸烟大鼠的实验性牙周炎模型。方法：采用被动吸烟结合结扎丝结扎法制备吸烟大鼠实验性牙周炎模型。健康Wistar大鼠40只随机分为健康对照组(C)、单纯结扎组(L)、单纯吸烟组(S)、吸烟+结扎组(SL),每组均为10只。C组正常喂养,不做任何干预；L组以双股结扎丝结扎上颌第二磨牙牙颈部；S组每天放入吸烟染毒箱被动吸烟2次,12支/次,30min/次。SL组以双股结扎丝结扎上颌第二磨牙牙颈部,每天与S组同时给予被动吸烟。每周观察牙龈健康状况,当实验组大鼠出现明显的牙龈炎症,且探诊深度明显加深时处死动物,通过大体及X线检查观察并记录上颌第二磨牙牙龈指数(GI)、牙周探诊深度(PD)和牙槽骨丧失值(ABL),通过组织学检查观察牙槽骨变化,并通过酶联免疫标记法测定血清和牙龈组织中IL-6含量。结果：第6周时,SL组探诊深度明显增加。L组除PD和血清中IL-6含量,其余指标均高于C,S组。SL组各观察值高于S,L组。S组血清中IL-6含量高于C,L组。第8周时,L组探诊深度明显增加。L组、SL组牙龈指数、牙周探诊深度、牙槽骨丧失值、牙龈中IL-6含量均高于C,S组。而SL、L组GI无明显差别,余各观察值均以SL组高。S组、SL组血清IL-6高于C,L组。结论：1、被动吸烟+结扎丝结扎法可成功建立吸烟大鼠实验性牙周炎模型。2、第6周时可以形成稳定的吸烟大鼠牙周炎模型。
[Abstract]:Objective: to observe the changes of periodontal tissue in the neck of maxillary second molar of smoking rats with ligation of silk and to establish the experimental periodontitis model of smoking rats. Methods: the experimental periodontitis model of smoking rats was established by passive smoking combined with ligation of silk. Forty healthy Wistar rats were randomly divided into three groups: healthy control group (C), simple ligation group (L), smoking group (S) and smoking ligation group (SL). Each group was fed with 10 normal rats. Group S was put into a smoking box twice a day for passive smoking. Group SL was given passive smoking at the same time as group S by ligating the neck of maxillary second molar with double strands ligation wire twice a day. The gingival health status was observed every week. The rats in the experimental group were killed when the gingival inflammation was obvious and the depth of probing was obviously deepened. Gingival index (GI), periodontal probing depth (PD) and alveolar bone loss (ABL) of maxillary second molar were observed and recorded by gross and X-ray examination. The levels of IL-6 in serum and gingival tissue were determined by Elisa. Results: at the 6th week, the depth of probing in the SL group was significantly higher than that in the control group except PD and IL-6 content in serum, and the other indexes were higher than those in the CUSS group and the SL group. The serum IL-6 level in the S group was higher than that in the Con L group and the serum IL-6 level in the S group was higher than that in the Cell-L group. At the 8th week, the probing depth, gingival index, periodontal probing depth, alveolar bone loss and IL-6 content in the gingiva of the L group were significantly higher than those of the CUSS group. However, there was no significant difference in GI in SLL group, and the other observed values were higher in SL group than in Cell-L group. Conclusion the experimental periodontitis model of smoking rats can be established successfully by the method of ligation of silk by passive smoking, and a stable periodontitis model of smoking rats can be established at the 6th week.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R781.4;R-332

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