富勒醇对基于悬滴培养的脂肪间充质干细胞成骨分化的影响

发布时间：2018-07-04 17:31

  本文选题：间质干细胞 + 细胞分化　； 参考：《解放军医学杂志》2016年08期

【摘要】：目的探讨富勒醇对基于悬滴培养的大鼠脂肪间充质干细胞(r ADSCs)向成骨细胞分化的影响。方法将rADSCs通过悬滴培养3d形成大鼠脂肪间充质干细胞球,将细胞球用胰酶消化分散成单细胞,对获得的单细胞进行二维贴壁培养24h,然后更换为成骨诱导培养基培养,其中未添加富勒醇组为对照组,添加1.0μmol/L富勒醇组为实验组,诱导分化14d及21d后利用茜素红染色和实时定量PCR检测脂肪干细胞球来源的单细胞向成骨细胞分化的能力。结果悬滴培养3d的rADSCs可形成大小均一的微球结构,经胰酶消化分散可获得细胞球来源的单细胞。与对照组相比,在成骨诱导培养基中添加1.0μmol/L富勒醇可使所获单细胞形成更多的矿化的钙结节,且相关成骨基因Runx2、OCN、ColⅠ的表达增强。结论富勒醇可以显著增强基于悬滴培养获得的rADSCs的成骨分化,有助于提高其成骨诱导的效率。
[Abstract]:Objective to investigate the effect of fullethanol on differentiation of rat adipose mesenchymal stem cells (r ADSCs) into osteoblasts. Methods Rat adipose mesenchymal stem cell spheres were formed by suspension culture of rADSCs for 3 days. The cells were digested and dispersed into single cells by trypsin. The obtained cells were cultured for 24 hours with two-dimensional adherent culture and then replaced with osteoblast induction medium. The control group was treated with no fullethanol and the experimental group with 1.0 渭 mol / L fulleol. After 14 and 21 days of differentiation induction, alizarin red staining and real-time quantitative PCR were used to detect the ability of single cells derived from adipose stem cells to differentiate into osteoblasts. Results the rADSCs cultured for 3 days could form a uniform structure of microspheres, and a single cell derived from the cells could be obtained by trypsin digestion and dispersing. Compared with the control group, adding 1.0 渭 mol / L fulleol to the osteoblast induction medium resulted in the formation of more mineralized calcium nodules and increased expression of the osteogenic gene Runx2OCNCol 鈪，

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