温阳益气活血方对冠心病大鼠模型FGF-2及VEGF表达的影响

发布时间：2018-07-04 19:59

本文选题：冠状动脉性心脏病 + 心功能　；参考：《青海医药杂志》2016年11期

【摘要】：目的:观察温阳益气活血方对冠心病大鼠模型心肌组织FGF-2及VEGF表达的影响。方法:40只SD大鼠随机分为正常组、假手术组、模型组和中药组,每组10只。采用左冠状动脉前降支中上1/3处行结扎法建立冠心病大鼠模型,造模一周后给予药物干预,共28天。治疗结束后测心功能及冠状动脉血流量,然后处死动物,取左室心肌组织,采用RT-FQ-PCR检测FGF-2及VEGF mRNA的表达水平。结果:模型组LVSP、+dp/dtmax及-dp/dtmax的绝对值及冠脉血流量较对照组和假手术组明显下降,差异具有统计学意义(P0.05)。与模型组比较,中药组LVSP、+dp/dtmax、-dp/dtmax的绝对值及冠脉血流量增高,差异具有统计学意义(P0.05)。模型组FGF-2和VEGF mRNA表达水平较正常组和假手术组明显升高(P0.05),中药组FGF-2和VEGF mRNA表达水平均较模型组明显升高(P0.05);空白组和假手术组相比,差异没有统计学意义(P0.05)。结论:温阳益气活血方具有通过上调FGF-2和VEGF基因表达促进心肌新生血管的形成,改善心肌供血和提高心功能的作用。
[Abstract]:Aim: to observe the effect of Wenyang Yiqi Huoxue recipe on the expression of FGF-2 and VEGF in myocardial tissue of coronary heart disease (CHD) rats. Methods 40 Sprague-Dawley rats were randomly divided into normal group, sham operation group, model group and traditional Chinese medicine group with 10 rats in each group. The rat model of coronary artery disease (CHD) was established by ligating the left anterior descending coronary artery at the middle and upper third of the left coronary artery for 28 days. Cardiac function and coronary artery blood flow were measured after treatment. The left ventricular myocardium was taken from the animals and the expression levels of FGF-2 and VEGF mRNA were detected by RT-FQ-PCR. Results: the absolute values of LVSP, dp/dtmax and -dp / DT max and coronary blood flow in the model group were significantly lower than those in the control group and the sham operation group (P0.05). Compared with the model group, the absolute value of LVSP, DP / dtmax-dp / dtmax and coronary blood flow were significantly higher in TCM group (P0.05). The expression of FGF-2 and VEGF mRNA in the model group was significantly higher than that in the normal group and sham-operation group (P0.05), and the expression level of FGF-2 and VEGF mRNA in the traditional Chinese medicine group was significantly higher than that in the model group (P0.05), but there was no significant difference between the blank group and the sham-operation group (P0.05). Conclusion: Wenyang Yiqi Huoxue recipe can promote myocardial neovascularization by up-regulation of FGF-2 and VEGF gene expression, improve myocardial blood supply and improve cardiac function.
【作者单位】：青海省中医院心肾科;
【分类号】：R285.5;R-332

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