IL34转基因小鼠的建立和表型分析

发布时间：2018-07-05 20:22

本文选题：白介素 + 转基因小鼠　；参考：《中国比较医学杂志》2011年06期

【摘要】：目的建立白介素34转基因小鼠,研究该基因在小鼠中表达对小鼠免疫系统的作用。方法把人的IL34基因插入CMV启动子下,构建转基因表达载体,通过显微注射法建立IL34转基因C57BL/6J小鼠。PCR鉴定IL34转基因小鼠的基因表型,Western blotting检测IL34蛋白的表达水平,组织化学染色观察IL34转基因小鼠重要器官的病理改变。结果建立了2个品系的IL34转基因小鼠。转入的人IL34基因在脾脏中的表达水平高于内源性IL34。组织学分析显示IL34转基因小鼠各重要器官如脑,心,肝,肾,肠等的形态结构均正常,但脾脏的生发中心比较活跃,白髓的范围比野生型小鼠大。结论成功建立了IL34转基因小鼠,IL34基因的过度表达对免疫系统作用需要进一步探讨。
[Abstract]:Objective to study the effect of interleukin 34 gene expression on immune system in mice. Methods Human IL-34 gene was inserted into CMV promoter to construct transgenic expression vector. The gene phenotype of IL-34 transgenic C57BL / 6J mice was determined by microinjection. The expression level of IL-34 protein was detected by Western blotting. The pathological changes of important organs of IL 34 transgenic mice were observed by histochemical staining. Results two strains of IL 34 transgenic mice were established. The expression level of human IL34 gene in spleen was higher than that in endogenous IL 34 gene. Histological analysis showed that the morphology and structure of important organs such as brain, heart, liver, kidney and intestine of IL-34 transgenic mice were normal, but the germinal center of spleen was active, and the range of white pulp was larger than that of wild type mice. Conclusion the overexpression of IL-34 gene in IL-34 transgenic mice needs further study.
【作者单位】：中国医学科学院北京协和医学院医学实验动物研究所卫生部人类疾病比较医学重点实验室国家中医药管理局人类疾病动物模型三级实验室;
【基金】：卫生部项目,实验动物和人类疾病动物模型资源扩展(200802036) 十一五新药专项支持(2009ZX09501-026)
【分类号】：R-332

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