小鼠细粒棘球蚴体外促血管生成作用研究

发布时间：2018-07-06 21:01

本文选题：细粒棘球蚴 + 血管生成　；参考：《中国血吸虫病防治杂志》2017年03期

【摘要】：目的分析细粒棘球蚴体外对人脐静脉内皮细胞血管生成能力的影响及其促血管生成因子转录水平,初步探讨细粒棘球蚴的促血管生成作用。方法将继发感染小鼠体内的细粒棘球蚴体外培养后的上清作用于体外培养的人脐静脉内皮细胞,观察其成管现象并用NIH Image J软件的Angiogenesis模块进行分析。同时,在细粒棘球蚴基因组中寻找同宿主(小鼠)的基质金属蛋白酶9(Matrix metalloproteinase-9,MMP-9)和高迁移率族蛋白B1(High mobility group box B1,HMGB1)的同源蛋白,并对其转录水平进行分析。结果鼠源细粒棘球蚴囊培养上清能显著促进人脐静脉内皮细胞的成管(F=73.03,P0.001),在细粒棘球蚴囊壁和原头节中存在小鼠MMP-9和HMGB1同源蛋白的表达,且MMP-9在原头节中的转录水平高于棘球蚴囊(t=-11.65,P0.001),而HMGB1在棘球蚴囊中的转录水平高于原头节(t=6.43,P=0.003)。结论细粒棘球蚴囊培养上清可能含有促进血管生成的虫源分子,但其确切机制还有待进一步研究。
[Abstract]:Objective to analyze the effect of Echinococcus granulosus on angiogenesis in human umbilical vein endothelial cells in vitro and the transcription level of angiogenic factor, and to investigate the angiogenesis effect of Echinococcus granulosus. Methods the upper culture of Echinococcus granulosus in the body of secondary infection mice was acted on the human umbilical vein endothelial cells cultured in vitro. Cells were observed and analyzed with the Angiogenesis module of NIH Image J software. At the same time, the homoprotein of matrix metalloproteinase 9 (Matrix metalloproteinase-9, MMP-9) and high mobility group protein B1 (High mobility group box) was found in the genome of Echinococcus granulosus and its transcriptional level. Results the supernatant of Echinococcus granulosus sac can significantly promote the F=73.03 (P0.001) of human umbilical vein endothelial cells (F=73.03, P0.001), and the expression of MMP-9 and HMGB1 homologous protein in the cyst wall and the proto joint of the Echinococcus granulosus, and the transcription level of MMP-9 in the original joint is higher than that of the echinococcosis (t=-11.65, P0.001), and HMGB1 is in the spinous ball. The transcriptional level in the sac is higher than that of the original joint (t=6.43, P=0.003). Conclusion the supernatant of Echinococcus granulosus culture may contain the insect source molecules that promote angiogenesis, but the exact mechanism remains to be further studied.
【作者单位】：中国疾病预防控制中心寄生虫病预防控制所、卫生部寄生虫病原与媒介生物学重点实验室、世界卫生组织热带病合作中心、科技部国家级热带病国际联合研究中心;
【基金】：国家自然科学基金(81371842、81371841) 上海市三年行动计划第四轮重点学科(15GWZK0101)
【分类号】：R383.33

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