徐长卿-二妙散-三藤方对蛋白聚糖诱导关节炎模型小鼠血清中TNF-α和DKK-1含量的影响

发布时间：2018-07-07 14:08

本文选题：徐长卿-二妙散-三藤方 + 关节炎　；参考：《中国药房》2017年31期

【摘要】：目的:研究徐长卿-二妙散-三藤方对关节炎模型小鼠血清中肿瘤坏死因子α(TNF-α)和骨化相关因子DKK-1含量的影响,探讨该方治疗关节炎的作用机制。方法:将60只BALB/c小鼠随机分为正常组、模型组、柳氮磺吡啶组(阳性对照,9 mg/kg)和徐长卿-二妙散-三藤方低、中、高剂量组(以生药量计分别为11.25、22.5、45 g/kg),每组10只。除正常组外的其余50只小鼠均采用完全弗氏佐剂+蛋白聚糖ip法建立关节炎模型。成模后,各给药组小鼠ig相应药物,正常组和模型组小鼠ig等体积生理盐水,每天1次,连续20 d。给药结束后,采用酶联免疫吸附法检测各组小鼠血清中TNF-α和DKK-1含量,透射电镜下观察小鼠骶髂关节滑膜细胞超微病理结构变化。结果:与正常组比较,模型组小鼠血清中TNF-α含量明显升高、DKK-1含量明显降低(P0.05);镜下可见小鼠骶髂关节滑膜细胞发生增生肥大、胞质内细胞器变形、线粒体肿胀等病理损伤。与模型组比较,各给药组小鼠血清中TNF-α含量均明显降低(P0.05或P0.01),除柳氮磺吡啶组外的其余各给药组小鼠血清中DKK-1含量均明显升高(P0.05);镜下可见各给药组小鼠骶髂关节滑膜细胞的病理损伤均有不同程度减轻,且徐长卿-二妙散-三藤方组小鼠的病理损伤好转程度较柳氮磺吡啶组更显著。结论:徐长卿-二妙散-三藤方可能通过降低血清中TNF-α含量和升高DKK-1含量,从而抑制关节炎模型小鼠的骶髂关节炎症和病理性骨化。
[Abstract]:Aim: to study the effect of Xuchang Qing-Ermiao San-Santeng recipe on the contents of tumor necrosis factor-伪 (TNF- 伪) and ossification related factor (DKK-1) in the serum of arthritis model mice, and to explore the mechanism of its therapeutic effect on arthritis. Methods: sixty BALB / c mice were randomly divided into normal group, model group, salicylazosulfopyridine group (positive control group, 9 mg/kg) and low, medium and high dose groups (11.25U 22.545 g/kg, respectively) with 10 rats in each group. All the other 50 mice except the normal group were treated with complete Freund's adjuvant proteoglycan IP method to establish arthritis model. After the model was established, the mice of each administration group were given the corresponding drugs, and the normal group and the model group were given the same volume of normal saline once a day for 20 days. After administration, the levels of TNF- 伪 and DKK-1 in serum of each group were detected by enzyme-linked immunosorbent assay (Elisa), and the ultrastructural changes of synovial cells in sacroiliac joint of mice were observed under transmission electron microscope. Results: compared with the normal group, the serum TNF- 伪 content in the model group was significantly increased (P0.05), and the synovial cells of the sacroiliac joint were hyperplastic, cytoplasmic organelle deformed, mitochondria swelling and other pathological damage. Compared with the model group, The content of TNF- 伪 in the serum of each administration group was significantly decreased (P0.05 or P0.01), and the serum DKK-1 content of the other groups except salicylazosulfopyridine group was significantly increased (P0.05), and the pathological changes of synovial cells of sacroiliac joint of mice in each group were observed under microscope. The damage was alleviated to some extent, The pathological damage of mice in Xuchang Qing-Ermiao San-San Fufang group was more obvious than that in sulfasalopyridine group. Conclusion: Xuchang Qing-Ermiao San-Santeng decoction may inhibit sacroiliac arthritis and pathological ossification by decreasing the content of TNF- 伪 and increasing the content of DKK-1 in serum.
【作者单位】：湖北中医药大学临床技能实训中心;湖北中医药大学针灸骨伤学院;湖北中医药大学第一临床学院;
【基金】：湖北省教育厅科学研究计划资助项目(No.Q20162011)
【分类号】：R285.5;R-332

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