益肾通癃胶囊对前列腺增生模型小鼠生殖激素水平的影响

发布时间：2018-07-07 17:17

  本文选题：益肾通癃胶囊 + 良性前列腺增生　； 参考：《中华男科学杂志》2016年11期

【摘要】：目的:探讨益肾通癃胶囊对良性前列腺增生(BPH)模型小鼠生殖激素水平的影响。方法:对SPF雄性小鼠皮下注射丙酸睾酮5mg/(kg·d),连续3周,待造模成功后分为6组:正常对照组,模型组,癃闭舒胶囊组,益肾通癃胶囊高、中、低剂量组,每组10只。益肾通癃胶囊高、中、低剂量组分别用1.2,0.6,0.3 g/kg益肾通癃胶囊混悬液灌胃,癃闭舒胶囊组用0.45 g/kg癃闭舒胶囊混悬液灌胃,正常对照组及模型组给予同体积蒸馏水。给药8周后处死小鼠,称取前列腺湿重并计算前列腺指数(PI),测量小鼠血清中睾酮(T)、雌二醇(E2)的水平、E2/T比值,光镜下观察小鼠前列腺组织的形态变化。结果:与模型组比较,益肾通癃胶囊中、高剂量组及癃闭舒胶囊组均可降低模型小鼠前列腺湿重、PI指数(P均0.01)。各组小鼠血清T、E2、E2/T水平:正常对照组:(1.73±0.02)ng/ml、(73.08±1.03)pg/ml、42.30±0.53;模型组:(3.86±0.02)ng/ml,(145.79±0.88)pg/ml,37.76±0.25;癃闭舒胶囊组:(2.47±0.02)ng/ml,(95.87±0.47)pg/ml,38.80±0.13;益肾通癃胶囊高剂量组:(2.75±0.03)ng/ml,(107.11±0.61)pg/ml,38.92±0.36;益肾通癃胶囊中剂量组:(2.77±0.02)ng/ml,(112.16±0.82)pg/ml,40.56±0.29;益肾通癃胶囊低剂量组:(2.91±0.03)ng/ml,(112.68±0.77)pg/ml,38.80±0.42,各组与模型组相比,差异均具有统计学意义(P0.05或P0.01)。结论:益肾通癃胶囊可能通过降低BPH模型小鼠血清T、E2,升高E2/T比值,揭示了益肾通癃胶囊治疗BPH的机制。
[Abstract]:Objective: To investigate the effect of Yishen Tong Long capsule (Yishen Tong Long capsule) on the level of reproductive hormone in mice with benign prostatic hyperplasia (BPH). Methods: SPF male mice were subcutaneously injected with testosterone propionate 5mg/ (kg. D) for 3 weeks. After the model was successful, the model group was divided into 6 groups: normal control group, model group, yishanshu capsule group, Yishen Tong Long capsule high, medium and low dose group, 10 rats in each group. Yishen Tong Long capsule high, medium and low dose group were fed with 1.2,0.6,0.3 g/kg Yishen Tong Long capsule suspension, respectively, and long Shu Capsule group was fed with 0.45 g/kg long long closed capsule suspension. The normal control group and the model group were given the same volume of distilled water. After 8 weeks of administration, the mice were killed and the prostatic index (PI) was measured and the mice were measured, and the mice were measured. The level of serum testosterone (T), estradiol (E2), E2/T ratio, and the morphological changes of the prostate tissue in mice were observed under light microscope. Results: compared with the model group, the high dose group and the long closed capsule group could reduce the wet weight of the prostate in the model mice and the number of PI (P 0.01). The serum T, E2, E2/T level of the mice in each group: (1.7 3 + 0.02) ng/ml, (73.08 + 1.03) pg/ml, 42.30 + 0.53, model group: (3.86 + 0.02) ng/ml, (145.79 + 0.88) pg/ml, 37.76 + 0.25, (2.47 + 0.02) ng/ml, (2.47 + 0.53) ng/ml, high dose group of Yishen Tong Long capsule: ng/ml /ml, (112.16 + 0.82) pg/ml, 40.56 + 0.29, Yishen Tong Long capsule low dose group: (2.91 + 0.03) ng/ml, (112.68 + 0.77) pg/ml, 38.80 + 0.42, the difference was statistically significant compared with the model group (P0.05 or P0.01). Conclusion: Yishen Tong Long capsule may reduce the serum T, E2, and E2/T ratio of BPH model mice, and reveal Yishen Tong Long glue The mechanism of the cysts for the treatment of BPH.
【作者单位】： 湖南中医药大学第一附属医院中医外科;湖南中医药大学研究生院;
【基金】：湖南中医药管理局重点科研项目(201510)~~
【分类号】：R277.5;R-332
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本文编号：2105670