瑞舒伐他汀钙对老年脓毒症模型大鼠急性肺损伤的干预作用

发布时间：2018-07-08 14:13

本文选题：脓毒症 + 急性肺损伤　；参考：《中国老年学杂志》2017年11期

【摘要】：目的探讨瑞舒伐他汀钙对老年脓毒症大鼠急性肺损伤的干预作用。方法将72只老年雄性Wistar大鼠随机分为假手术组、模型组、瑞舒伐他汀钙干预组,每组24只。干预组及模型组采用改良盲肠结扎穿孔术方法建立老年脓毒症ALI大鼠模型,假手术组建立假手术模型。术前1 w干预组开始每日给予瑞舒伐他汀钙,剂量2.5 mg·kg~(-1)·d~(-1)。模型组和假手术组同一时间给予等量生理盐水。实验前24 h灌注药物后禁食,自由饮水。每组大鼠平均分为3个亚组,分别于术后6、12和24 h取材。应用酶联免疫吸附法(ELISA)检测血清细胞因子白细胞介素(IL)-2、IL-6、肿瘤坏死因子(TNF)-α水平、检测动脉血氧分压(PaO_2)并观察肺组织的病理改变。结果模型组PaO_2明显低于假手术组和干预组(P0.05),且随时间延长而降低。模型组血清细胞因子IL-2、IL-6、TNF-α水平高于假手术组和干预组(P0.05),与模型组比,干预组肺损伤程度较轻。结论瑞舒伐他汀钙能抑制老年脓毒症大鼠ALI的炎症反应、改善氧分压,进而减轻ALI。
[Abstract]:Objective to investigate the effect of rosuvastatin calcium on acute lung injury in aged sepsis rats. Methods Seventy-two aged male Wistar rats were randomly divided into sham-operation group, model group and rosuvastatin calcium intervention group with 24 rats in each group. The intervention group and the model group established the model of senile sepsis Ali by modified cecal ligation and perforation, and the sham operation group established the sham-operation model. The intervention group was given recuvastatin calcium daily at a dose of 2.5 mg kg ~ (-1) d ~ (-1). The model group and the sham operation group were given the same amount of saline at the same time. The drug was infused 24 hours before the experiment and fasting and drinking water was free. The rats in each group were divided into 3 subgroups on average. The samples were obtained at 6 h and 24 h postoperatively. The levels of serum interleukin (IL)-2 and tumor necrosis factor-伪 (TNF-伪) were measured by enzyme linked immunosorbent assay (Elisa). The arterial partial pressure of oxygen (Pao) was measured and the pathological changes of lung tissue were observed. Results PaO2 in model group was significantly lower than that in sham operation group and intervention group (P0.05), and decreased with time. The level of serum cytokine IL-6 TNF- 伪 in the model group was higher than that in the sham operation group and the intervention group (P0.05). Compared with the model group, the degree of lung injury in the intervention group was less than that in the control group. Conclusion Risuvastatin calcium can inhibit the inflammatory response of Ali in aged sepsis rats, improve the partial pressure of oxygen, and then alleviate the ALI.
【作者单位】：复旦大学附属金山医院急诊医学科;
【分类号】：R-332;R459.7

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